GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Sample GSM67375

Query DataSets for GSM67375

Status

Public on Sep 26, 2005

Title

del337TR replicate 7

Sample type

RNA

Source name

Mouse heart

Organism

Mus musculus

Characteristics

deletion in 337TR

Extracted molecule

total RNA

Extraction protocol

Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

deletion in 337TR

Data processing

Robust Multichip Analysis (RMA)

Submission date

Aug 05, 2005

Last update date

Sep 26, 2005

Contact name

Norman Buroker

E-mail(s)

Norman.Buroker@seattlechildrens.org

Organization name

Seattle Children's Hospital

Lab

Portman

Street address

4800 Sand Point Way N.E.

City

Seattle

State/province

ZIP/Postal code

98105

Country

USA

Platform ID

GPL1261

Series (1)

GSE3067

Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	231.1	P	0.000754
AFFX-BioB-M_at	341.8	P	0.00034
AFFX-BioB-3_at	201.1	P	0.000662
AFFX-BioC-5_at	601.4	P	0.00011
AFFX-BioC-3_at	518.3	P	0.00007
AFFX-BioDn-5_at	730.1	P	0.000044
AFFX-BioDn-3_at	3469.3	P	0.000081
AFFX-CreX-5_at	8425.2	P	0.000044
AFFX-CreX-3_at	10059.4	P	0.000044
AFFX-DapX-5_at	6.4	A	0.672921
AFFX-DapX-M_at	38.7	A	0.239024
AFFX-DapX-3_at	59.9	A	0.354441
AFFX-LysX-5_at	5.2	A	0.699409
AFFX-LysX-M_at	47.1	A	0.382589
AFFX-LysX-3_at	27.1	A	0.185089
AFFX-PheX-5_at	1215.5	P	0.000044
AFFX-PheX-M_at	1563.7	P	0.000052
AFFX-PheX-3_at	2862.8	P	0.000044
AFFX-ThrX-5_at	7.2	A	0.937071
AFFX-ThrX-M_at	2	A	0.978107

Total number of rows: 45101

Table truncated, full table size 1205 Kbytes.

Supplementary data files not provided