GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM67376 Query DataSets for GSM67376
Status Public on Sep 26, 2005
Title Control with WY14643 replicate 1
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics Control treated with WY14643
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Control treated with WY14643
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 228.8 P 0.000753
AFFX-BioB-M_at 458.2 P 0.000127
AFFX-BioB-3_at 206.1 P 0.000225
AFFX-BioC-5_at 552.2 P 0.000169
AFFX-BioC-3_at 350.1 P 0.00007
AFFX-BioDn-5_at 566.1 P 0.000081
AFFX-BioDn-3_at 2624 P 0.000297
AFFX-CreX-5_at 7582.9 P 0.000052
AFFX-CreX-3_at 9144.3 P 0.000044
AFFX-DapX-5_at 18.7 A 0.165861
AFFX-DapX-M_at 22 A 0.368438
AFFX-DapX-3_at 39.2 M 0.062929
AFFX-LysX-5_at 10914.5 P 0.000044
AFFX-LysX-M_at 13010.4 P 0.000044
AFFX-LysX-3_at 12872.6 P 0.000044
AFFX-PheX-5_at 3.3 A 0.922016
AFFX-PheX-M_at 4.3 A 0.891021
AFFX-PheX-3_at 28.7 A 0.51489
AFFX-ThrX-5_at 4.6 A 0.957038
AFFX-ThrX-M_at 4.1 A 0.749204

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap