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Sample GSM67380 Query DataSets for GSM67380
Status Public on Sep 26, 2005
Title Control with WY14643 replicate 5
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics Control treated with WY14643
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Control treated with WY14643
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 236.4 P 0.000857
AFFX-BioB-M_at 492.3 P 0.000225
AFFX-BioB-3_at 231.1 P 0.00039
AFFX-BioC-5_at 652.3 P 0.000127
AFFX-BioC-3_at 552 P 0.000052
AFFX-BioDn-5_at 745.9 P 0.000052
AFFX-BioDn-3_at 3592.2 P 0.000095
AFFX-CreX-5_at 9098.6 P 0.000052
AFFX-CreX-3_at 10064.5 P 0.000044
AFFX-DapX-5_at 13.7 A 0.108979
AFFX-DapX-M_at 25.2 A 0.313723
AFFX-DapX-3_at 36.4 A 0.250796
AFFX-LysX-5_at 9810.7 P 0.000044
AFFX-LysX-M_at 13806.6 P 0.000044
AFFX-LysX-3_at 14319.4 P 0.000044
AFFX-PheX-5_at 2.2 A 0.852061
AFFX-PheX-M_at 1.4 A 0.957038
AFFX-PheX-3_at 10.5 A 0.645547
AFFX-ThrX-5_at 5 A 0.724854
AFFX-ThrX-M_at 14.7 A 0.455413

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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