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Sample GSM67383 Query DataSets for GSM67383
Status Public on Sep 26, 2005
Title del337 with WY14643 replicate 1
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics del337 treated with WY14643
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description del337 treated with WY14643
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 174.9 P 0.002556
AFFX-BioB-M_at 237.9 P 0.000446
AFFX-BioB-3_at 128.3 P 0.011384
AFFX-BioC-5_at 384.4 P 0.000225
AFFX-BioC-3_at 298.5 P 0.00011
AFFX-BioDn-5_at 435 P 0.00007
AFFX-BioDn-3_at 2731.1 P 0.000258
AFFX-CreX-5_at 5183.8 P 0.000044
AFFX-CreX-3_at 6003.5 P 0.000044
AFFX-DapX-5_at 28.5 A 0.083592
AFFX-DapX-M_at 47.7 A 0.095667
AFFX-DapX-3_at 76.1 P 0.042962
AFFX-LysX-5_at 14 A 0.440646
AFFX-LysX-M_at 57.6 A 0.425962
AFFX-LysX-3_at 21.9 A 0.067678
AFFX-PheX-5_at 1.9 A 0.987453
AFFX-PheX-M_at 1.7 A 0.971543
AFFX-PheX-3_at 22 A 0.368438
AFFX-ThrX-5_at 11.6 A 0.804734
AFFX-ThrX-M_at 3.4 A 0.910522

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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