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Sample GSM67384 Query DataSets for GSM67384
Status Public on Sep 26, 2005
Title del337 with WY14643 replicate 2
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics del337 treated with WY14643
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description del337 treated with WY14643
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 272.5 P 0.000857
AFFX-BioB-M_at 502.8 P 0.000258
AFFX-BioB-3_at 226.7 P 0.000509
AFFX-BioC-5_at 666.4 P 0.000127
AFFX-BioC-3_at 535.8 P 0.000044
AFFX-BioDn-5_at 725.2 P 0.000044
AFFX-BioDn-3_at 3930.8 P 0.000095
AFFX-CreX-5_at 9132.9 P 0.000052
AFFX-CreX-3_at 10490.8 P 0.000044
AFFX-DapX-5_at 37.3 M 0.062929
AFFX-DapX-M_at 75.3 P 0.033677
AFFX-DapX-3_at 87.9 P 0.020022
AFFX-LysX-5_at 11 A 0.425962
AFFX-LysX-M_at 30.3 A 0.60308
AFFX-LysX-3_at 35.9 P 0.039661
AFFX-PheX-5_at 2 A 0.953518
AFFX-PheX-M_at 2.6 A 0.945787
AFFX-PheX-3_at 40.3 A 0.239063
AFFX-ThrX-5_at 4.2 A 0.953518
AFFX-ThrX-M_at 2.1 A 0.749204

Total number of rows: 45101

Table truncated, full table size 1205 Kbytes.

Supplementary data files not provided

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