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Sample GSM67385 Query DataSets for GSM67385
Status Public on Sep 26, 2005
Title del337 with WY14643 replicate 3
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics del337 treated with WY14643
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description del337 treated with WY14643
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 425.3 P 0.000857
AFFX-BioB-M_at 651.7 P 0.000147
AFFX-BioB-3_at 289.2 P 0.00011
AFFX-BioC-5_at 975.5 P 0.000081
AFFX-BioC-3_at 740.6 P 0.000044
AFFX-BioDn-5_at 986.1 P 0.000044
AFFX-BioDn-3_at 4872 P 0.000081
AFFX-CreX-5_at 12168.2 P 0.000052
AFFX-CreX-3_at 13925.2 P 0.000044
AFFX-DapX-5_at 2.9 A 0.672935
AFFX-DapX-M_at 36 A 0.195266
AFFX-DapX-3_at 50.9 A 0.0727
AFFX-LysX-5_at 14.9 A 0.455413
AFFX-LysX-M_at 35.6 A 0.354453
AFFX-LysX-3_at 43 P 0.001796
AFFX-PheX-5_at 2 A 0.904333
AFFX-PheX-M_at 2.1 A 0.984817
AFFX-PheX-3_at 14.2 A 0.48511
AFFX-ThrX-5_at 4.4 A 0.876428
AFFX-ThrX-M_at 12.4 A 0.631562

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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