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Sample GSM67387 Query DataSets for GSM67387
Status Public on Sep 26, 2005
Title del337 with WY14643 replicate 5
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics del337 treated with WY14643
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description del337 treated with WY14643
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 301.3 P 0.000754
AFFX-BioB-M_at 412.8 P 0.000258
AFFX-BioB-3_at 218.2 P 0.000127
AFFX-BioC-5_at 646.7 P 0.000169
AFFX-BioC-3_at 607.3 P 0.000044
AFFX-BioDn-5_at 952.8 P 0.000052
AFFX-BioDn-3_at 3743.6 P 0.000147
AFFX-CreX-5_at 10302.6 P 0.000052
AFFX-CreX-3_at 9295.6 P 0.000044
AFFX-DapX-5_at 33.1 P 0.046482
AFFX-DapX-M_at 56.4 P 0.036569
AFFX-DapX-3_at 69.3 P 0.026111
AFFX-LysX-5_at 2.4 A 0.686277
AFFX-LysX-M_at 25.9 A 0.5
AFFX-LysX-3_at 21.5 P 0.042962
AFFX-PheX-5_at 1.7 A 0.953518
AFFX-PheX-M_at 2.5 A 0.937071
AFFX-PheX-3_at 11.1 A 0.544587
AFFX-ThrX-5_at 5.5 A 0.737173
AFFX-ThrX-M_at 7.1 A 0.617401

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided

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