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Sample GSM67389 Query DataSets for GSM67389
Status Public on Sep 26, 2005
Title del337 with WY14643 replicate 7
Sample type RNA
Source name Mouse heart
Organism Mus musculus
Characteristics del337 treated with WY14643
Extracted molecule total RNA
Extraction protocol Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description del337 treated with WY14643
Data processing Robust Multichip Analysis (RMA)
Submission date Aug 05, 2005
Last update date Sep 26, 2005
Contact name Norman Buroker
Organization name Seattle Children's Hospital
Lab Portman
Street address 4800 Sand Point Way N.E.
City Seattle
State/province WA
ZIP/Postal code 98105
Country USA
Platform ID GPL1261
Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 244.3 P 0.001102
AFFX-BioB-M_at 411.4 P 0.000509
AFFX-BioB-3_at 187 P 0.010317
AFFX-BioC-5_at 674 P 0.00011
AFFX-BioC-3_at 490.6 P 0.000044
AFFX-BioDn-5_at 759.2 P 0.00007
AFFX-BioDn-3_at 3769.2 P 0.000147
AFFX-CreX-5_at 9619.2 P 0.000052
AFFX-CreX-3_at 10988.7 P 0.000044
AFFX-DapX-5_at 30.1 A 0.262736
AFFX-DapX-M_at 46 A 0.354453
AFFX-DapX-3_at 75.4 P 0.020022
AFFX-LysX-5_at 1.3 A 0.868847
AFFX-LysX-M_at 44.5 A 0.52976
AFFX-LysX-3_at 20.8 A 0.165861
AFFX-PheX-5_at 3.4 A 0.910522
AFFX-PheX-M_at 2.5 A 0.979978
AFFX-PheX-3_at 27.1 A 0.574038
AFFX-ThrX-5_at 33.4 A 0.60308
AFFX-ThrX-M_at 5.2 A 0.824672

Total number of rows: 45101

Table truncated, full table size 1206 Kbytes.

Supplementary data files not provided

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