|
Status |
Public on Oct 04, 2005 |
Title |
third instar larvae -4 hours relative to pupariation EcRi mutant replicate 1 |
Sample type |
RNA |
|
|
Source name |
whole animal
|
Organism |
Drosophila melanogaster |
Characteristics |
Transgenic animal containing a heat-inducible transgene that expresses a double-stranded RNA corresponding to the EcR common region (hs-EcRi-11)
|
Treatment protocol |
hs-EcRi-11 third instar larvae were heat-treated twice at 37°C, each time for 1 hour, at 24 hours and 18 hours prior to pupariation
|
Growth protocol |
Larvae were staged by addition of 0.05% bromophenol blue and collected as actively wandering larvae with clear guts
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from 10 animals using TriPure (Roche) followed by further purification with RNAeasy columns (Qiagen)
|
Label |
biotin
|
Label protocol |
Approxiamately 12 ug of total RNA was processed to produce biotinylated cRNA targets
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
EcR mutants die during early stages of development, complicating their use for studying receptor function during metamorphosis. To circumvent this problem, we employed a transgenic system that allows heat-induced expression of double-stranded RNA corresponding to the EcR common region to disrupt EcR function at puparium formation (EcRi)
|
Data processing |
dChip 1.2
|
|
|
Submission date |
Aug 05, 2005 |
Last update date |
Sep 02, 2005 |
Contact name |
Robert B Beckstead |
E-mail(s) |
robertb@uga.edu
|
Phone |
706-542-0387
|
Fax |
706-542-1827
|
Organization name |
The University of Georgia
|
Department |
Poultry Science
|
Lab |
Robert B. Beckstead
|
Street address |
119 Poultry Science Building
|
City |
Athens |
State/province |
GA |
ZIP/Postal code |
30602-2772 |
Country |
USA |
|
|
Platform ID |
GPL72 |
Series (1) |
GSE3069 |
Identification of genes dependent on the Ecdysone receptor (EcR) at the onset of metamorphosis in Drosophila |
|