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Sample GSM67399 Query DataSets for GSM67399
Status Public on Oct 04, 2005
Title third instar larvae -4 hours relative to pupariation EcRi mutant replicate 1
Sample type RNA
 
Source name whole animal
Organism Drosophila melanogaster
Characteristics Transgenic animal containing a heat-inducible transgene that expresses a double-stranded RNA corresponding to the EcR common region (hs-EcRi-11)
Treatment protocol hs-EcRi-11 third instar larvae were heat-treated twice at 37°C, each time for 1 hour, at 24 hours and 18 hours prior to pupariation
Growth protocol Larvae were staged by addition of 0.05% bromophenol blue and collected as actively wandering larvae with clear guts
Extracted molecule total RNA
Extraction protocol RNA was isolated from 10 animals using TriPure (Roche) followed by further purification with RNAeasy columns (Qiagen)
Label biotin
Label protocol Approxiamately 12 ug of total RNA was processed to produce biotinylated cRNA targets
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description EcR mutants die during early stages of development, complicating their use for studying receptor function during metamorphosis. To circumvent this problem, we employed a transgenic system that allows heat-induced expression of double-stranded RNA corresponding to the EcR common region to disrupt EcR function at puparium formation (EcRi)
Data processing dChip 1.2
 
Submission date Aug 05, 2005
Last update date Sep 02, 2005
Contact name Robert B Beckstead
E-mail(s) robertb@uga.edu
Phone 706-542-0387
Fax 706-542-1827
Organization name The University of Georgia
Department Poultry Science
Lab Robert B. Beckstead
Street address 119 Poultry Science Building
City Athens
State/province GA
ZIP/Postal code 30602-2772
Country USA
 
Platform ID GPL72
Series (1)
GSE3069 Identification of genes dependent on the Ecdysone receptor (EcR) at the onset of metamorphosis in Drosophila

Data table header descriptions
ID_REF
VALUE dChip derived PM-only model gene expression

Data table
ID_REF VALUE
142121_at 392.37
142122_at 229.84
142123_at 280.54
142124_at 33.33
142125_at 49.72
142126_at 125.98
142127_at 78.11
142128_at 94.5
142129_at 56.26
142130_at 209.4
142131_at 119.97
142132_at 271.88
142133_at 439.02
142134_at 373.78
142135_at 58.9
142136_at 368.73
142137_at 151.94
142138_at 330.86
142139_at 529.46
142140_at 421.32

Total number of rows: 13966

Table truncated, full table size 229 Kbytes.




Supplementary file Size Download File type/resource
GSM67399.CEL.CEL.gz 2.8 Mb (ftp)(http) CEL

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