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Sample GSM674023 Query DataSets for GSM674023
Status Public on Feb 20, 2012
Title rc194_T00317395
Sample type RNA
 
Source name Exfoliated colonocytes
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
diet: FO/P
injection: AOM
time: 24 h after AOM injection
Treatment protocol Rats consumed diets containing FO/P or CO/C and were injected with azoxymethane (AOM, 2x, 15 mg/kg BW, s.c.).
Growth protocol Rats were housed individually in a temperature and humidity controlled animal facility with a 12 h light/dark cycle.
Extracted molecule total RNA
Extraction protocol To enrich the level of eukaryotic mRNA in the fecal samples, poly (A)+ RNA was isolated from total RNA using oligo dT cellulose micro spin columns and the mTRAP Maxi kit.
Label biotin
Label protocol Ambion MessageAmp II aRNA Amplification and Biotin Enhanced kits: Manual Labelled cRNA Target preparation following manufacturer's instructions http://www.ambion.com/techlib/prot/fm_1751.pdf and http://www.ambion.com/techlib/prot/fm_1791.pdf
 
Hybridization protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
Scan protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
Description T00317395_2011-2-1_RC 194 24h
Data processing Median normalized values derived from Codelink Expression Analysis Version 5.0
 
Submission date Feb 12, 2011
Last update date Feb 20, 2012
Contact name Youngmi Cho
E-mail(s) ym473@tamu.edu
Phone 979-845-4887
Organization name Texas A&M University
Department Faculty of Nutrition
Street address 2253 TAMU
City College Station
State/province TX
ZIP/Postal code 77840
Country USA
 
Platform ID GPL2896
Series (1)
GSE27266 Fish oil/pectin temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis

Data table header descriptions
ID_REF
VALUE Median normalized values (signals) derived from Codelink Expression Analysis Version 5.0

Data table
ID_REF VALUE
1001 299.2915
1002 1.8847
1004 2.2448
1005 94.5735
1006 8.1838
1007 284.6389
1008 258.8604
1009 4.951
1010 0.2647
1011 1.9817
1012 1.1909
1013 4.7133
1014 283.7638
1015 274.584
1016 0.8268
1017 0.3279
1018 0.8422
1019 1.4377
1020 28.2344
1021 278.8589

Total number of rows: 35974

Table truncated, full table size 481 Kbytes.




Supplementary file Size Download File type/resource
GSM674023.TXT.gz 1.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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