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Sample GSM674032 Query DataSets for GSM674032
Status Public on Feb 20, 2012
Title ir574_T00317396
Sample type RNA
 
Source name Exfoliated colonocytes
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
diet: FO/P
injection: AOM
time: 24 h after AOM injection
Treatment protocol Rats consumed diets containing FO/P or CO/C and were injected with azoxymethane (AOM, 2x, 15 mg/kg BW, s.c.).
Growth protocol Rats were housed individually in a temperature and humidity controlled animal facility with a 12 h light/dark cycle.
Extracted molecule total RNA
Extraction protocol To enrich the level of eukaryotic mRNA in the fecal samples, poly (A)+ RNA was isolated from total RNA using oligo dT cellulose micro spin columns and the mTRAP Maxi kit.
Label biotin
Label protocol Ambion MessageAmp II aRNA Amplification and Biotin Enhanced kits: Manual Labelled cRNA Target preparation following manufacturer's instructions http://www.ambion.com/techlib/prot/fm_1751.pdf and http://www.ambion.com/techlib/prot/fm_1791.pdf
 
Hybridization protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
Scan protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
Description T00317396_2011-2-1_IR574 24h
Data processing Median normalized values derived from Codelink Expression Analysis Version 5.0
 
Submission date Feb 12, 2011
Last update date Feb 20, 2012
Contact name Youngmi Cho
E-mail(s) ym473@tamu.edu
Phone 979-845-4887
Organization name Texas A&M University
Department Faculty of Nutrition
Street address 2253 TAMU
City College Station
State/province TX
ZIP/Postal code 77840
Country USA
 
Platform ID GPL2896
Series (1)
GSE27266 Fish oil/pectin temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis

Data table header descriptions
ID_REF
VALUE Median normalized values (signals) derived from Codelink Expression Analysis Version 5.0

Data table
ID_REF VALUE
1001 245.0307
1002 0.7964
1004 0.4771
1005 39.3226
1006 11.4163
1007 239.451
1008 197.5139
1009 3.7779
1010 0.657
1011 0.3597
1012 3.1229
1013 7.9106
1014 228.05
1015 216.0401
1016 0.4003
1017 0.5322
1018 0.3425
1019 1.4742
1020 10.2037
1021 216.7359

Total number of rows: 35974

Table truncated, full table size 482 Kbytes.




Supplementary file Size Download File type/resource
GSM674032.TXT.gz 1.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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