NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM674039 Query DataSets for GSM674039
Status Public on Feb 20, 2012
Title ir884_T00316332
Sample type RNA
 
Source name Exfoliated colonocytes
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
diet: CO/C
injection: AOM
time: 24 h after AOM injection
Treatment protocol Rats consumed diets containing FO/P or CO/C and were injected with azoxymethane (AOM, 2x, 15 mg/kg BW, s.c.).
Growth protocol Rats were housed individually in a temperature and humidity controlled animal facility with a 12 h light/dark cycle.
Extracted molecule total RNA
Extraction protocol To enrich the level of eukaryotic mRNA in the fecal samples, poly (A)+ RNA was isolated from total RNA using oligo dT cellulose micro spin columns and the mTRAP Maxi kit.
Label biotin
Label protocol Ambion MessageAmp II aRNA Amplification and Biotin Enhanced kits: Manual Labelled cRNA Target preparation following manufacturer's instructions http://www.ambion.com/techlib/prot/fm_1751.pdf and http://www.ambion.com/techlib/prot/fm_1791.pdf
 
Hybridization protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
Scan protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
Description T00316332_2011-2-1_IR884 24h
Data processing Median normalized values derived from Codelink Expression Analysis Version 5.0
 
Submission date Feb 12, 2011
Last update date Feb 20, 2012
Contact name Youngmi Cho
E-mail(s) ym473@tamu.edu
Phone 979-845-4887
Organization name Texas A&M University
Department Faculty of Nutrition
Street address 2253 TAMU
City College Station
State/province TX
ZIP/Postal code 77840
Country USA
 
Platform ID GPL2896
Series (1)
GSE27266 Fish oil/pectin temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis

Data table header descriptions
ID_REF
VALUE Median normalized values (signals) derived from Codelink Expression Analysis Version 5.0

Data table
ID_REF VALUE
1001 666.9458
1002 1.0674
1004 1.0428
1005 116.2197
1006 7.8176
1007 635.3972
1008 634.5108
1009 5.221
1010 0.4523
1011 0.6708
1012 1.1063
1013 11.6607
1014 608.5716
1015 589.4437
1016 1.0161
1017 1.1275
1018 0.4151
1019 0.6873
1020 29.3839
1021 620.9367

Total number of rows: 35974

Table truncated, full table size 484 Kbytes.




Supplementary file Size Download File type/resource
GSM674039.TXT.gz 1.7 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap