|
| Status |
Public on Oct 04, 2005 |
| Title |
4 hour prepupae EcR mutant replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
whole animal
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Transgenic animal containing a heat-inducible transgene that expresses a double-stranded RNA corresponding to the EcR common region (hs-EcRi-11)
|
| Treatment protocol |
hs-EcRi-11 third instar larvae were heat-treated twice at 37°C, each time for 1 hour, at 24 hours and 18 hours prior to pupariation
|
| Growth protocol |
Prepupae were staged relative to pupariation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 10 animals using TriPure (Roche) followed by further purification with RNAeasy columns (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Approxiamately 12 ug of total RNA was processed to produce biotinylated cRNA targets
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
EcR mutants die during early stages of development, complicating their use for studying receptor function during metamorphosis. To circumvent this problem, we employed a transgenic system that allows heat-induced expression of double-stranded RNA corresponding to the EcR common region to disrupt EcR function at puparium formation (EcRi)
|
| Data processing |
dChip 1.2
|
| |
|
| Submission date |
Aug 05, 2005 |
| Last update date |
Sep 02, 2005 |
| Contact name |
Robert B Beckstead |
| E-mail(s) |
robertb@uga.edu
|
| Phone |
706-542-0387
|
| Fax |
706-542-1827
|
| Organization name |
The University of Georgia
|
| Department |
Poultry Science
|
| Lab |
Robert B. Beckstead
|
| Street address |
119 Poultry Science Building
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602-2772 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE3069 |
Identification of genes dependent on the Ecdysone receptor (EcR) at the onset of metamorphosis in Drosophila |
|
