|
| Status |
Public on Feb 20, 2012 |
| Title |
RCT0171_T00320414 |
| Sample type |
RNA |
| |
|
| Source name |
Exfoliated colonocytes
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
diet: FO/P
injection: AOM
time: 28 wk after AOM injection
|
| Treatment protocol |
Rats consumed diets containing FO/P or CO/C and were injected with azoxymethane (AOM, 2x, 15 mg/kg BW, s.c.).
|
| Growth protocol |
Rats were housed individually in a temperature and humidity controlled animal facility with a 12 h light/dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
To enrich the level of eukaryotic mRNA in the fecal samples, poly (A)+ RNA was isolated from total RNA using oligo dT cellulose micro spin columns and the mTRAP Maxi kit.
|
| Label |
biotin
|
| Label protocol |
Ambion MessageAmp II aRNA Amplification and Biotin Enhanced kits: Manual Labelled cRNA Target preparation following manufacturer's instructions http://www.ambion.com/techlib/prot/fm_1751.pdf and http://www.ambion.com/techlib/prot/fm_1791.pdf
|
| |
|
| Hybridization protocol |
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
|
| Scan protocol |
Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
|
| Description |
T00320414_2011-2-1_RCT171 28WK
|
| Data processing |
Median normalized values derived from Codelink Expression Analysis Version 5.0
|
| |
|
| Submission date |
Feb 12, 2011 |
| Last update date |
Feb 20, 2012 |
| Contact name |
Youngmi Cho |
| E-mail(s) |
ym473@tamu.edu
|
| Phone |
979-845-4887
|
| Organization name |
Texas A&M University
|
| Department |
Faculty of Nutrition
|
| Street address |
2253 TAMU
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77840 |
| Country |
USA |
| |
|
| Platform ID |
GPL2896 |
| Series (1) |
| GSE27266 |
Fish oil/pectin temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis |
|
