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Status
Public on Aug 05, 2006
Title
KC control
Sample type
RNA
Source name
tissue culture, embryo-derived cells, female, control
Organism
Drosophila melanogaster Characteristics
Wild type, Cell Type Kc167, control
Biomaterial provider
DGRC, Drosophila Genomics Resource Center
Treatment protocol
Kc167 cells were diluted to 1x10^6 cells/ml in CCM3 media and plated in a 150mm culture dish. All samples were incubated for 72 hours, mock-treated, allowed to rest for 90 minutes, and then prepped for total RNA.
Growth protocol
Kc167 cells were maintained in serum-free CCM3 media supplemented with 1:200 dilution of Penicilin-Streptomycin (Gibco) at 25 degrees C.
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted using Trizol reagent (Gibco/BRL).
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Kc167 cells, control
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Aug 05, 2005
Last update date
Aug 05, 2005
Contact name
John M Abrams
E-mail(s)
John.Abrams@UTSouthwestern.edu Phone
214-648-9226
Fax
214-648-8694
Organization name
University of Texas Southwestern Medical Center
Department
Cell Biology
Lab
Abrams
Street address
5323 Harry Hines Blvd
City
Dallas
State/province
TX
ZIP/Postal code
75390-9039
Country
USA
Platform ID
GPL72 Series (1)
GSE3072 Ecdysone- and stress-induced expression profile in Kc167 cells
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE 141200_at
16.3
A
0.849855
141201_at
96.9
A
0.703191
141202_at
363.9
A
0.078906
141203_at
179
A
0.216313
141204_at
396.8
A
0.135974
141205_at
60.7
A
0.703191
141206_at
309.7
P
0.017732
141207_at
230.6
A
0.110449
141208_at
243.2
A
0.078854
141209_at
313.7
P
0.03202
141210_at
127.3
A
0.437646
141211_at
171.8
A
0.437646
141212_at
826.8
P
0.020663
141213_at
22.1
A
0.96798
141214_at
283.2
A
0.216313
141215_at
384.2
A
0.150145
141216_at
64.8
A
0.635055
141217_at
476.7
A
0.296809
141218_at
212.3
A
0.099061
141219_at
175.1
A
0.586954
14010 360 Kbytes .Supplementary file Size Download File type/resource GSM67489.CEL.gz
2.4 Mb
(ftp) (http) CEL
GSM67489.EXP.EXP.gz
460 b
(ftp) (http) EXP
