|
| Status |
Public on Aug 05, 2006 |
| Title |
KC gamma-irradiated |
| Sample type |
RNA |
| |
|
| Source name |
tissue culture, embryo-derived cells, female, gamma-irradiated
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Wild type, Cell Type Kc167, gamma-irradiated
|
| Biomaterial provider |
DGRC, Drosophila Genomics Resource Center
|
| Treatment protocol |
Kc167 cells were diluted to 1x10^6 cells/ml in CCM3 media and plated in a 150mm culture dish. All samples were incubated for 72 hours, gamma-irradiated at 280 Gy, allowed to rest for 90 minutes, and then prepped for total RNA.
|
| Growth protocol |
Kc167 cells were maintained in serum-free CCM3 media supplemented with 1:200 dilution of Penicilin-Streptomycin (Gibco) at 25 degrees C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol reagent (Gibco/BRL).
|
| Label |
biotin
|
| Label protocol |
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Kc167 cells, gamma-irradiated
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Aug 05, 2005 |
| Last update date |
Aug 05, 2005 |
| Contact name |
John M Abrams |
| E-mail(s) |
John.Abrams@UTSouthwestern.edu
|
| Phone |
214-648-9226
|
| Fax |
214-648-8694
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Department |
Cell Biology
|
| Lab |
Abrams
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9039 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE3072 |
Ecdysone- and stress-induced expression profile in Kc167 cells |
|
