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Sample GSM67553 Query DataSets for GSM67553
Status Public on Aug 31, 2006
Title mut 20 rep1
Sample type RNA
 
Source name upf1- cell at time point 20
Organism Saccharomyces cerevisiae
Characteristics Yeast strain S288C with UPF1 ORF replaced by KanMX4. Cells harvested at 20 minutes after transcription inhibition.
Extracted molecule total RNA
Extraction protocol hot phenol protocol
Label biotin
Label protocol cDNA was synthesized from 15ug total RNA from all samples using oligo(dT) primer. 5ul out of 12ul RnaseH-puriÞed cDNA samples were used to synthesize biotinylated cRNA.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Yeast cultures were grown to mid-log phase and harvested at 20 minutes after transcription inhibition by 10ug/ml thiolutin.
Data processing Robust Multiarray Average
 
Submission date Aug 08, 2005
Last update date Feb 21, 2006
Contact name Michael Culbertson
E-mail(s) mrculber@wisc.edu
Phone 608-262-5388
Fax 608-262-4570
Organization name University of Wisconsin
Department Genetics
Street address 1525 Linden Drive
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platform ID GPL90
Series (1)
GSE3076 Impact of Nonsense-mediated mRNA Decay on the Global Expression Profile of Budding Yeast

Data table header descriptions
ID_REF probe ID
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
10000_at 2.1002
10001_at 7.1209
10002_i_at 10.2106
10003_f_at 11.0438
10004_at 3.0164
10005_at 6.7558
10006_at 5.7134
10007_at 8.5671
10008_at 5.1964
10009_at 6.1473
10010_at 8.6207
10011_at 3.1515
10012_at 6.5612
10013_at 3.5432
10014_at 7.7495
10015_at 9.6721
10016_at 8.8041
10017_at 4.2214
10018_at 11.854
10019_at 10.1804

Total number of rows: 9335

Table truncated, full table size 141 Kbytes.




Supplementary file Size Download File type/resource
GSM67553.CEL.gz 1.8 Mb (ftp)(http) CEL
GSM67553.CHP.gz 2.5 Mb (ftp)(http) CHP
GSM67553.EXP.gz 444 b (ftp)(http) EXP
Processed data provided as supplementary file

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