|
| Status |
Public on Aug 31, 2006 |
| Title |
mut 20 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
upf1- cell at time point 20
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Yeast strain S288C with UPF1 ORF replaced by KanMX4. Cells harvested at 20 minutes after transcription inhibition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
hot phenol protocol
|
| Label |
biotin
|
| Label protocol |
cDNA was synthesized from 15ug total RNA from all samples using oligo(dT) primer. 5ul out of 12ul RnaseH-puriÞed cDNA samples were used to synthesize biotinylated cRNA.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Yeast cultures were grown to mid-log phase and harvested at 20 minutes after transcription inhibition by 10ug/ml thiolutin.
|
| Data processing |
Robust Multiarray Average
|
| |
|
| Submission date |
Aug 08, 2005 |
| Last update date |
Feb 21, 2006 |
| Contact name |
Michael Culbertson |
| E-mail(s) |
mrculber@wisc.edu
|
| Phone |
608-262-5388
|
| Fax |
608-262-4570
|
| Organization name |
University of Wisconsin
|
| Department |
Genetics
|
| Street address |
1525 Linden Drive
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE3076 |
Impact of Nonsense-mediated mRNA Decay on the Global Expression Profile of Budding Yeast |
|
