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Sample GSM67603 Query DataSets for GSM67603
Status Public on Aug 31, 2006
Title wt 30 rep1
Sample type RNA
 
Source name upf1- cell at time point 30
Organism Saccharomyces cerevisiae
Characteristics Yeast strain S288C with wild type UPF1 ORF. Cells harvested at 30 minutes after transcription inhibition.
Extracted molecule total RNA
Extraction protocol hot phenol protocol
Label biotin
Label protocol cDNA was synthesized from 15ug total RNA from all samples using oligo(dT) primer. 5ul out of 12ul RnaseH-puriÞed cDNA samples were used to synthesize biotinylated cRNA.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Yeast cultures were grown to mid-log phase and harvested at 30 minutes after transcription inhibition by 10ug/ml thiolutin.
Data processing Robust Multiarray Average
 
Submission date Aug 08, 2005
Last update date Feb 21, 2006
Contact name Michael Culbertson
E-mail(s) mrculber@wisc.edu
Phone 608-262-5388
Fax 608-262-4570
Organization name University of Wisconsin
Department Genetics
Street address 1525 Linden Drive
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platform ID GPL90
Series (1)
GSE3076 Impact of Nonsense-mediated mRNA Decay on the Global Expression Profile of Budding Yeast

Data table header descriptions
ID_REF probe ID
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
10000_at 2.2951
10001_at 7.7269
10002_i_at 9.7662
10003_f_at 10.3974
10004_at 3.2806
10005_at 6.8251
10006_at 4.949
10007_at 8.9748
10008_at 5.2152
10009_at 5.9227
10010_at 8.7424
10011_at 3.3081
10012_at 7.4761
10013_at 4.3213
10014_at 7.7695
10015_at 9.516
10016_at 9.5891
10017_at 4.549
10018_at 12.1925
10019_at 9.4521

Total number of rows: 9335

Table truncated, full table size 141 Kbytes.




Supplementary file Size Download File type/resource
GSM67603.CEL.gz 1.8 Mb (ftp)(http) CEL
GSM67603.CHP.gz 2.5 Mb (ftp)(http) CHP
GSM67603.EXP.gz 454 b (ftp)(http) EXP
Processed data provided as supplementary file

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