|
| Status |
Public on Feb 18, 2011 |
| Title |
db/db mouse sample 111 |
| Sample type |
RNA |
| |
|
| Source name |
24 week db/db mouse sciatic nerve
|
| Organism |
Mus musculus |
| Characteristics |
tissue: sciatic nerve
strain: BKS
genotype/variation: db/db
age: 24 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from sciatic nerve using a commercially available silica gel-based isolation protocol (RNeasy Mini Kit, QIAGEN Inc., Valencia, CA) including an on-column DNase digestion following the manufacturer's protocol. RNA quantity and quality was measured by microfluid electrophoresis using the RNA 6000 Pico LabChip® on a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Of the total RNA, 75 ng from each sample was amplified and biotin-labeled using the Ovation™ Biotin-RNA Amplification and Labeling System (NuGEN Technologies Inc., San Carlos, CA) according to the manufacturer’s protocol
|
| |
|
| Hybridization protocol |
Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
|
| Description |
Gene expression data from 24 week db/db mouse sciatic nerve
|
| Data processing |
The Affymetrix CEL files were initially analyzed using a local version of the GenePattern genomic analysis platform from the Broad Institute. The samples were Robust Multi-array Average (RMA).
|
| |
|
| Submission date |
Feb 17, 2011 |
| Last update date |
Jan 06, 2012 |
| Contact name |
Manjusha Pande |
| E-mail(s) |
mpande@umich.edu
|
| Organization name |
University of Michigan
|
| Department |
BRCF
|
| Lab |
Bioinformatics Core
|
| Street address |
2800 Plymouth Rd.
|
| City |
Ann Arobr |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL9746 |
| Series (2) |
| GSE27382 |
Transcriptional Profiling of Diabetic Neuropathy in BKS-db/db Mouse, a Model for Type 2 Diabetes |
| GSE34889 |
Molecular Mechanisms of Diabetic Neuropathy: A Transcriptional Profling Study of db/db Mouse Sciatic Nerve |
|
