|
| Status |
Public on Feb 23, 2011 |
| Title |
IM_vehicle_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney inner medulla collected from vehicle treated control rat
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: kidney inner medulla
gender: male
body weight: 200–250 g
|
| Treatment protocol |
For vehicle (saline, 1 µl/h, s.c. for 5 d) or dDAVP (40 ng/h, s.c. for 5 d, D5d) infusion, osmotic minipumps were implanted subcutaneously in the neck of each rat. osmotic minipumps were removed from rats under light enflurane inhalation anesthesia and rats were awakened, returned to metabolic cages, and kept for 3hr (D5d-3h).
|
| Growth protocol |
Pathogen-free male Sprague-Dawley rats were obtained from Charles River. The animal protocols were approved by the Animal Care and Use Committee of Kyungpook National University, and all animal experiments were conducted according to the guidelines of Kyungpook National University. Vehicle-treated control rats and dDAVP-treated rats were maintained in metabolic cages. All animals were maintained on a fixed amount of standard rodent diet (20 g/220 g BW/day) with free access to water intake.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The Affymetrix GeneChip® procedure for generation of the biotin-labeled single-stranded cDNA by in vitro transcription were performed according to the manufacturer's instructions from 300ng total RNA(GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Rev.5). Protocol performed using the Whole Transcript Sense Target Labeling and Control Reagents (Affymetrix 900652).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of framented cDNA were hybridized for about 17 hr at 45℃ on Rat Gene 1.0 ST array. The hybridized probe arrays were stained and washed with GeneChip® Hybridization, Wash and Stain Kit using the Fluidics Station 450(Affymetrix).
|
| Scan protocol |
The stained GeneChips were scanned with a GeneChip® Scanner 3000+ 7G(Affymetrix) a 570 nm.
|
| Description |
Gene expression data at the control state
|
| Data processing |
The RMA algorithm was used for expression summary and signal calculation of Affymetrix Rat Gene 1.0 ST array data. Sketch Quantile normalization was performed for the signal normalization.
|
| |
|
| Submission date |
Feb 22, 2011 |
| Last update date |
Feb 23, 2011 |
| Contact name |
Tae-Hwan Kwon |
| E-mail(s) |
thkwon@knu.ac.kr
|
| Phone |
+82 53 420 4825
|
| Fax |
+82 53 422 1466
|
| Organization name |
School of Medicine, Kyungpook National University
|
| Department |
Department of Biochemistry and Cell Biology
|
| Street address |
Dongin-dong 101
|
| City |
Taegu |
| ZIP/Postal code |
700-422 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE27449 |
Changes of mRNA expressin in rat kidney inner medulla in response to dDAVP stimulation/ withdrawal |
|
