|
| Status |
Public on Feb 26, 2011 |
| Title |
LA mouse desipramine hippocampus 2 |
| Sample type |
RNA |
| |
|
| Source name |
Murine hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
strain: Swiss-Webster
tissue: hippocampus
age: 15 weeks
phenotype: low swim stress-induced analgesia
treatment: desipramine
|
| Treatment protocol |
The animals received 21 intraperitoneal injections of desipramine hydrochloride (7.5 mg/kg/day).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using NucleoSpin RNA II kit (Macherey-Nagel, Germany), separately from each hippocampus according to the manufacturer’s protocol. Quantity and quality of each RNA sample was estimated by Nanodrop (Nanodrop, USA) and Bioanalyzer (Agilent, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina RNA Amplification Kit (Ambion Inc., USA) according to the manufacturer’s protocol and starting with 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalized using quantile method preceded by log2 transformation with functions provided by beadarray package, part of Bioconductor project.
|
| |
|
| Submission date |
Feb 25, 2011 |
| Last update date |
Feb 26, 2011 |
| Contact name |
Pawel Lisowski |
| E-mail(s) |
pawel.lisowski@mdc-berlin.de
|
| Organization name |
Max-Delbrück-Center for Molecular Medicine (MDC) in the Helmholtz Association
|
| Street address |
Robert-Rössle-Str. 10
|
| City |
Berlin |
| ZIP/Postal code |
13092 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE27532 |
Effects of chronic desipramine treatment on hippocampal transcriptome in mice differing in response to antidepressants |
|
