|
Status |
Public on Feb 26, 2011 |
Title |
LA mouse saline hippocampus 3 |
Sample type |
RNA |
|
|
Source name |
Murine hippocampus
|
Organism |
Mus musculus |
Characteristics |
strain: Swiss-Webster tissue: hippocampus age: 15 weeks phenotype: low swim stress-induced analgesia treatment: saline
|
Treatment protocol |
The animals received 21 intraperitoneal injections of desipramine hydrochloride (7.5 mg/kg/day).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using NucleoSpin RNA II kit (Macherey-Nagel, Germany), separately from each hippocampus according to the manufacturer’s protocol. Quantity and quality of each RNA sample was estimated by Nanodrop (Nanodrop, USA) and Bioanalyzer (Agilent, USA).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared using the Illumina RNA Amplification Kit (Ambion Inc., USA) according to the manufacturer’s protocol and starting with 100 ng total RNA.
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 3
|
Data processing |
The data were normalized using quantile method preceded by log2 transformation with functions provided by beadarray package, part of Bioconductor project.
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|
|
Submission date |
Feb 25, 2011 |
Last update date |
Feb 26, 2011 |
Contact name |
Pawel Lisowski |
E-mail(s) |
pawel.lisowski@mdc-berlin.de
|
Organization name |
Max-Delbrück-Center for Molecular Medicine (MDC) in the Helmholtz Association
|
Street address |
Robert-Rössle-Str. 10
|
City |
Berlin |
ZIP/Postal code |
13092 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE27532 |
Effects of chronic desipramine treatment on hippocampal transcriptome in mice differing in response to antidepressants |
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