|
| Status |
Public on Nov 01, 2012 |
| Title |
Control 2 |
| Sample type |
RNA |
| |
|
| Source name |
HaCaT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HaCaT
cell type: keratinocytes
|
| Treatment protocol |
samples were exposed to cytokines at 200ng/ml for 12h
|
| Growth protocol |
HaCaT cells were grown in DMEM medium (1.5mM Ca), supplemented with 10% FCS, p/s and glu
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Plus Mini Ki
|
| Label |
biotin
|
| Label protocol |
biotinylation of cRNA
|
| |
|
| Hybridization protocol |
HumanHT12 v.4 Expression BeadChip, 750ng of bitinylated cRNA used
|
| Scan protocol |
Illumina iScan array scanner
|
| Description |
replicate 2
101069-02
|
| Data processing |
Illumina' Genomestudio v.2010.1 with default settings
|
| |
|
| Submission date |
Feb 25, 2011 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Stephen Taylor |
| E-mail(s) |
stephen.taylor@imm.ox.ac.uk
|
| Phone |
+44 1865 222640
|
| Organization name |
CBRG
|
| Street address |
Headington
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE27533 |
IL-17 downregulates filaggrin and affects epidermal barrier function gene expression; potential role in the pathogenesis of atopic eczema |
| GSE28158 |
IL-22 down-regulates filaggrin expression and affects expression of profilaggrin processing enzymes by keratinocytes |
|
