|
| Status |
Public on Feb 28, 2011 |
| Title |
MSI-4 |
| Sample type |
RNA |
| |
|
| Source name |
Colorectal, MSI
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: colorectal cancer
microsatellite phenotype: high-level microsatellite instability
hla expression: HLA-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen) + QIAshredder.
|
| Label |
Biotin
|
| Label protocol |
GeneChip 3’ IVT Express kit.
|
| |
|
| Hybridization protocol |
Samples were hybridized to Affymetrix HT HG-133+PM 24 Array Plate GeneChips using the Affymetrix standard hybridization protocol.
|
| Scan protocol |
Gene Titan System.
|
| Description |
MSI/HLA-
|
| Data processing |
Data were normalized using the RMA method included in the R Bioconductor package limma.
|
| |
|
| Submission date |
Feb 27, 2011 |
| Last update date |
Feb 28, 2011 |
| Contact name |
Francisco Ruiz-Cabello |
| Organization name |
Hospital Universitario Virgen de las Nieves
|
| Street address |
Avenida Fuerzas Armadas 2
|
| City |
Granada |
| ZIP/Postal code |
18014 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE27544 |
Genome-wide profiling characterizes CRCs with genetic instability and specific routes to HLA class I loss and immunoescape |
|
