|
| Status |
Public on Mar 01, 2011 |
| Title |
esco2 MO injected embryos at 24hpf, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
esco2 MO injected embryos at 24hpf
|
| Organism |
Danio rerio |
| Characteristics |
tissue: dechorionated whole embryos
genotype: esco2 morphants
age: 24 hpf
|
| Treatment protocol |
Zebrafish were injected at the 1-cell stage with either an esco2 morpholino (esco2 MO) or with buffer only (control).
|
| Growth protocol |
Embryos were kept in embryo medium at 28.5 degrees Celcius until collected for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 4 replicates (50 embryos each) using Trizol (Invitrogen) and purified using Qiagen RNeasy columns according to the manufacturer’s instructions. RNA integrity was measured using the Agilent Bioanalyzer 2100 (RIN ≥ 9.5 for all samples).
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was labeled and hybridized as outlined in the Affymetrix IVT express kit protocol catalogue number 901229. Arrays were washed using an Affymetrix 450 wash station and Affymetrix wash protocol FS450_0004
|
| |
|
| Hybridization protocol |
see label protocol
|
| Scan protocol |
Arrays were scanned using a 7Gplus Affymetrix scanner.
|
| Description |
Gene Expression data from esco2 morphant embryos at 24hpf
|
| Data processing |
Cell file data was generated using the Affymetrix Algorithm Feature extraction- 6.2.33. Data was normalized using RMAExpress.
|
| |
|
| Submission date |
Feb 28, 2011 |
| Last update date |
Mar 01, 2011 |
| Contact name |
Julia Horsfield |
| E-mail(s) |
julia.horsfield@otago.ac.nz
|
| Organization name |
University of Otago
|
| Department |
Pathology Department
|
| Lab |
Chromosome Structure and Development Group
|
| Street address |
Cnr Hanover and Great King Streets
|
| City |
Dunedin |
| ZIP/Postal code |
9016 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE27569 |
Expression data from zebrafish depleted of Esco2 |
|
