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Status |
Public on May 02, 2011 |
Title |
mouse_brown_adipose_cells treated with mir193b inhibitor |
Sample type |
RNA |
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|
Source name |
mouse primary brown preadipocytes, induction for 4 days
|
Organism |
Mus musculus |
Characteristics |
cell type: mouse_brown_adipose_cells treatment: mir193b inhibitor background: C57BL/6J gender: male age: 2 week
|
Treatment protocol |
Primary brown preadipocytes were isolated from young mice 2 weeks according to published methods (Cannon and Nedergaard, 2001; Tseng et al., 2002). When cultured primary brown preadipocytes were at 70-80% confluence, Locked Nucleic Acids (LNAs) miRNA inhibitors (100nM) were transfected by DharmaFect 2 (6ul/ml) according to the manufacturer’s instruction. 24 hrs after transfection, cells were recovered in full culture media and grown to confluence for differentiation for 4 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using RNeasy kits (QIAGEN, Valencia, CA) according to the manufacturer's instructions. DNase (QIAGEN) was added to the RNeasy elution column as recommended by the manufacturer. RNA yield was quantified by UV spectrophotometry, and RNA integrity was verified using an Agilent Bioanalyzer.
|
Label |
biotin
|
Label protocol |
manufacturer's protocol
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|
|
Hybridization protocol |
manufacturer's protocol
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Scan protocol |
manufacturer's protocol
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Data processing |
Affymetrix array data were loaded into the R statistical environment with the affy package from BioConductor. Each probe was mapped to an Entrez Gene with R library mouse4302mmentrezgcdf, and genes were normalized with RMA.
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Submission date |
Mar 01, 2011 |
Last update date |
May 02, 2011 |
Contact name |
Bingbing Yuan |
E-mail(s) |
byuan@wi.mit.edu
|
Organization name |
Whitehead Institute
|
Street address |
9 Cambridge Center
|
City |
Cambridge |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL8492 |
Series (1) |
GSE27614 |
Expression data from mouse primary brown preadipocytes |
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