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Sample GSM684883 Query DataSets for GSM684883
Status Public on Feb 28, 2014
Title erlot2_CT41
Sample type RNA
 
Source name mouse, liver (CCl4), treated with erlotinib 2mg (week 18)
Organism Mus musculus
Characteristics tissue: liver
treatment: CCl4 and erlotinib 2mg
strain: A/J
Treatment protocol Animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” of the National Academy of Sciences. All animals were maintained in accordance with the institutional guidelines of the Massachusetts General Hospital Subcommittee on Research Animal Care. Strain A/J male mice (Jackson Laboratory, Bar Harbor, ME) were treated three times a week for 18 weeks with either 0.1cc of a 40 percent solution of CCl4 (Sigma) in olive oil or with vehicle control by oral gavage. Mice were sacrificed at 19 weeks after a one-week washout to eliminate acute effects of CCl4. The liver was sectioned and fixed in phosphate-buffered 10% formaldehyde for histological analysis. The remaining portions of the liver were collected in RNase-free tubes and snap-frozen in liquid nitrogen. Frozen tissues were stored at -80°C until use.
Extracted molecule total RNA
Extraction protocol Using frozen non-tumor liver tissues, total RNA was extracted using TRIzol (Invitrogen).
Label Cy5
Label protocol Refer to the Illumina beadarray Assay System Manual
 
Hybridization protocol Refer to the Illumina beadarray Assay System Manual
Scan protocol Refer to the Illumina beadarray Assay System Manual
Data processing For each probe, replicated mesurements (beads) were averaged after removing outliers. Data were were then normalized using the cubic spline algorithm.
 
Submission date Mar 02, 2011
Last update date Feb 28, 2014
Contact name Yujin Hoshida
E-mail(s) Yujin.Hoshida@UTSouthwestern.edu
Organization name University of Texas Southwestern Medical Center
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platform ID GPL6885
Series (2)
GSE27640 Gene expression profile of liver tissue in carbon tetrachloride (CCl4)-treated mouse treated with erlotinib
GSE27641 EGFR inhibition prevents liver cirrhosis and hepatocellular carcinoma

Data table header descriptions
ID_REF
VALUE Normalized signal intensities by GenePattern Illumina Normalizer module

Data table
ID_REF VALUE
ILMN_2896528 549.1890012
ILMN_2721178 308.2014408
ILMN_3033922 443.5413584
ILMN_3092673 1346.602617
ILMN_2816356 112.0463218
ILMN_2808939 321.7743942
ILMN_2634564 159.2783174
ILMN_2737647 111.7328456
ILMN_2734484 119.001056
ILMN_2952292 116.4956483
ILMN_2699078 111.4510996
ILMN_1213681 225.795422
ILMN_2735413 228.4965288
ILMN_2735415 297.0192124
ILMN_2891688 140.9924616
ILMN_2637698 135.173994
ILMN_2674228 176.5823416
ILMN_2601546 115.6982631
ILMN_1230831 112.6525694
ILMN_2848071 231.2261012

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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