|
| Status |
Public on Feb 28, 2014 |
| Title |
erlot2_CT42 |
| Sample type |
RNA |
| |
|
| Source name |
mouse, liver (CCl4), treated with erlotinib 2mg (week 18)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
treatment: CCl4 and erlotinib 2mg
strain: A/J
|
| Treatment protocol |
Animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” of the National Academy of Sciences. All animals were maintained in accordance with the institutional guidelines of the Massachusetts General Hospital Subcommittee on Research Animal Care. Strain A/J male mice (Jackson Laboratory, Bar Harbor, ME) were treated three times a week for 18 weeks with either 0.1cc of a 40 percent solution of CCl4 (Sigma) in olive oil or with vehicle control by oral gavage. Mice were sacrificed at 19 weeks after a one-week washout to eliminate acute effects of CCl4. The liver was sectioned and fixed in phosphate-buffered 10% formaldehyde for histological analysis. The remaining portions of the liver were collected in RNase-free tubes and snap-frozen in liquid nitrogen. Frozen tissues were stored at -80°C until use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using frozen non-tumor liver tissues, total RNA was extracted using TRIzol (Invitrogen).
|
| Label |
Cy5
|
| Label protocol |
Refer to the Illumina beadarray Assay System Manual
|
| |
|
| Hybridization protocol |
Refer to the Illumina beadarray Assay System Manual
|
| Scan protocol |
Refer to the Illumina beadarray Assay System Manual
|
| Data processing |
For each probe, replicated mesurements (beads) were averaged after removing outliers. Data were were then normalized using the cubic spline algorithm.
|
| |
|
| Submission date |
Mar 02, 2011 |
| Last update date |
Feb 28, 2014 |
| Contact name |
Yujin Hoshida |
| E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE27640 |
Gene expression profile of liver tissue in carbon tetrachloride (CCl4)-treated mouse treated with erlotinib |
| GSE27641 |
EGFR inhibition prevents liver cirrhosis and hepatocellular carcinoma |
|
