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Sample GSM684885 Query DataSets for GSM684885
Status Public on Feb 28, 2014
Title erlot5_CT36
Sample type RNA
 
Source name mouse, liver (CCl4), treated with erlotinib 5mg (week 18)
Organism Mus musculus
Characteristics tissue: liver
treatment: CCl4 and erlotinib 5 mg
strain: A/J
Treatment protocol Animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals” of the National Academy of Sciences. All animals were maintained in accordance with the institutional guidelines of the Massachusetts General Hospital Subcommittee on Research Animal Care. Strain A/J male mice (Jackson Laboratory, Bar Harbor, ME) were treated three times a week for 18 weeks with either 0.1cc of a 40 percent solution of CCl4 (Sigma) in olive oil or with vehicle control by oral gavage. Mice were sacrificed at 19 weeks after a one-week washout to eliminate acute effects of CCl4. The liver was sectioned and fixed in phosphate-buffered 10% formaldehyde for histological analysis. The remaining portions of the liver were collected in RNase-free tubes and snap-frozen in liquid nitrogen. Frozen tissues were stored at -80°C until use.
Extracted molecule total RNA
Extraction protocol Using frozen non-tumor liver tissues, total RNA was extracted using TRIzol (Invitrogen).
Label Cy5
Label protocol Refer to the Illumina beadarray Assay System Manual
 
Hybridization protocol Refer to the Illumina beadarray Assay System Manual
Scan protocol Refer to the Illumina beadarray Assay System Manual
Data processing For each probe, replicated mesurements (beads) were averaged after removing outliers. Data were were then normalized using the cubic spline algorithm.
 
Submission date Mar 02, 2011
Last update date Feb 28, 2014
Contact name Yujin Hoshida
E-mail(s) Yujin.Hoshida@UTSouthwestern.edu
Organization name University of Texas Southwestern Medical Center
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platform ID GPL6885
Series (2)
GSE27640 Gene expression profile of liver tissue in carbon tetrachloride (CCl4)-treated mouse treated with erlotinib
GSE27641 EGFR inhibition prevents liver cirrhosis and hepatocellular carcinoma

Data table header descriptions
ID_REF
VALUE Normalized signal intensities by GenePattern Illumina Normalizer module

Data table
ID_REF VALUE
ILMN_2896528 811.9259148
ILMN_2721178 378.8837734
ILMN_3033922 465.1953219
ILMN_3092673 1359.284234
ILMN_2816356 109.3847696
ILMN_2808939 310.4619248
ILMN_2634564 179.7949858
ILMN_2737647 107.5936525
ILMN_2734484 116.2872005
ILMN_2952292 117.7247024
ILMN_2699078 117.7247024
ILMN_1213681 220.8592913
ILMN_2735413 253.5375816
ILMN_2735415 272.9893458
ILMN_2891688 132.5853125
ILMN_2637698 151.4811394
ILMN_2674228 151.4811394
ILMN_2601546 114.1270422
ILMN_1230831 116.2872005
ILMN_2848071 255.7110105

Total number of rows: 25697

Table truncated, full table size 626 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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