NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM687660 Query DataSets for GSM687660
Status Public on May 30, 2011
Title Patient 404 treated 24h
Sample type RNA
 
Source name Patient 404 treated 24h
Organism Homo sapiens
Characteristics tumor type: chronic lymphocytic leukemia
tissue type: whole peripheral blood
therapeutic agent: SPC2996
dose group: 6mg per kg
time point: 24h post tx
Extracted molecule total RNA
Extraction protocol Whole PB samples were collected at the designated time points and stored at -80°C for up to 3 years in the PAXgene blood stabilizing system. After thawing, RNA was extracted employing the QIAGEN PAXgene extraction kit and repurified using the Rneasy MinElute Cleanup kit (QIAGEN).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 (G7 update).
Description Gene expression data from whole PB samples from CLL patient collected at the indicated time point after the first infusion of SPC2996
Data processing The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
 
Submission date Mar 09, 2011
Last update date May 30, 2011
Contact name Ludger Klein-Hitpass
E-mail(s) ludger.klein-hitpass@uni-essen.de
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
 
Platform ID GPL570
Series (1)
GSE27858 The novel antisense Bcl-2 inhibitor SPC2996 causes rapid leukemic cell clearance and immune activation in chronic lymphocytic leukemia

Data table header descriptions
ID_REF
VALUE ExpressionStat 5.0
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1569.81 P 0.000753643
AFFX-BioB-M_at 4245.75 P 5.16732e-05
AFFX-BioB-3_at 2461.2 P 0.000169227
AFFX-BioC-5_at 7631.69 P 5.16732e-05
AFFX-BioC-3_at 11776 P 5.16732e-05
AFFX-BioDn-5_at 13999.3 P 7.00668e-05
AFFX-BioDn-3_at 32364.8 P 4.42873e-05
AFFX-CreX-5_at 95726.3 P 5.16732e-05
AFFX-CreX-3_at 115829 P 4.42873e-05
AFFX-DapX-5_at 252.68 P 0.0103167
AFFX-DapX-M_at 746.131 P 0.0113844
AFFX-DapX-3_at 968.08 P 0.000753643
AFFX-LysX-5_at 2643.83 P 4.42873e-05
AFFX-LysX-M_at 4912.16 P 0.000258358
AFFX-LysX-3_at 10891.8 P 4.42873e-05
AFFX-PheX-5_at 10744 P 4.42873e-05
AFFX-PheX-M_at 13938.6 P 4.42873e-05
AFFX-PheX-3_at 12244.6 P 4.42873e-05
AFFX-ThrX-5_at 1752.26 P 9.4506e-05
AFFX-ThrX-M_at 2596.95 P 0.00010954

Total number of rows: 54675

Table truncated, full table size 1623 Kbytes.




Supplementary file Size Download File type/resource
GSM687660_404_24h.CEL.gz 4.9 Mb (ftp)(http) CEL
GSM687660_404_24h.CHP.gz 293.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap