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Sample GSM687716 Query DataSets for GSM687716
Status Public on May 30, 2011
Title Patient 449 treated 4h
Sample type RNA
 
Source name Patient 449 treated 4h
Organism Homo sapiens
Characteristics tumor type: chronic lymphocytic leukemia
tissue type: whole peripheral blood
therapeutic agent: SPC2996
dose group: 4mg per kg
time point: 4h post tx
Extracted molecule total RNA
Extraction protocol Whole PB samples were collected at the designated time points and stored at -80°C for up to 3 years in the PAXgene blood stabilizing system. After thawing, RNA was extracted employing the QIAGEN PAXgene extraction kit and repurified using the Rneasy MinElute Cleanup kit (QIAGEN).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133Plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 (G7 update).
Description Gene expression data from whole PB samples from CLL patient collected at the indicated time point after the first infusion of SPC2996
Data processing The data were analyzed with GCOS 1.4 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
 
Submission date Mar 09, 2011
Last update date May 30, 2011
Contact name Ludger Klein-Hitpass
E-mail(s) ludger.klein-hitpass@uni-essen.de
Phone +49 201 723 85552
Organization name Institut fuer Zellbiologie
Department Universitaetsklinikum
Lab BioChip Lab
Street address Virchowstr. 173
City Essen
ZIP/Postal code D-45122
Country Germany
 
Platform ID GPL570
Series (1)
GSE27858 The novel antisense Bcl-2 inhibitor SPC2996 causes rapid leukemic cell clearance and immune activation in chronic lymphocytic leukemia

Data table header descriptions
ID_REF
VALUE ExpressionStat 5.0
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1299.14 P 0.000856509
AFFX-BioB-M_at 1721.65 P 6.02111e-05
AFFX-BioB-3_at 1015.16 P 7.00668e-05
AFFX-BioC-5_at 4182.54 P 5.16732e-05
AFFX-BioC-3_at 5904.44 P 4.42873e-05
AFFX-BioDn-5_at 8957.16 P 4.42873e-05
AFFX-BioDn-3_at 18203.8 P 8.14279e-05
AFFX-CreX-5_at 52445.1 P 5.16732e-05
AFFX-CreX-3_at 57911.7 P 4.42873e-05
AFFX-DapX-5_at 344.206 P 0.000445901
AFFX-DapX-M_at 1100.58 P 0.00179591
AFFX-DapX-3_at 1167.6 P 0.000224668
AFFX-LysX-5_at 5221.11 P 4.42873e-05
AFFX-LysX-M_at 5771.93 P 4.42873e-05
AFFX-LysX-3_at 11688.9 P 4.42246e-05
AFFX-PheX-5_at 16183.3 P 4.42873e-05
AFFX-PheX-M_at 23798 P 4.42873e-05
AFFX-PheX-3_at 16846.8 P 4.42873e-05
AFFX-ThrX-5_at 2809.85 P 4.42873e-05
AFFX-ThrX-M_at 4531.68 P 4.42873e-05

Total number of rows: 54675

Table truncated, full table size 1636 Kbytes.




Supplementary file Size Download File type/resource
GSM687716_449_4h.CEL.gz 5.0 Mb (ftp)(http) CEL
GSM687716_449_4h.CHP.gz 292.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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