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Sample GSM692117 Query DataSets for GSM692117
Status Public on Mar 15, 2011
Title Donor 1 - CSE
Sample type RNA
Source name Human bronchial epithelial cells - 24 h CSE
Organism Homo sapiens
Characteristics donor: 1
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: CSE
Treatment protocol Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI = 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
Growth protocol Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
Extracted molecule total RNA
Extraction protocol Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
Label biotin
Label protocol Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
Hybridization protocol Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression in response to CSE exposure
Sample 1C
Data processing The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
Submission date Mar 15, 2011
Last update date Mar 15, 2011
Contact name David Proud
Organization name University of Calgary
Department Physiology & Pharmacology
Lab HRIC 4AC60
Street address 3330 Hospital DRive NW
City Calgary
State/province AB
ZIP/Postal code T2N 4N1
Country Canada
Platform ID GPL570
Series (1)
GSE27973 Human airway epithelial responses to rhinovirus infection and cigarette smoke extract alone and in combination

Data table header descriptions
VALUE MAS5.0 signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 1232.26 P 8.14279e-05
AFFX-BioB-M_at 2379.65 P 4.42873e-05
AFFX-BioB-3_at 1443.23 P 4.42873e-05
AFFX-BioC-5_at 3803.11 P 4.42873e-05
AFFX-BioC-3_at 3721.93 P 5.16732e-05
AFFX-BioDn-5_at 13996.7 P 4.42873e-05
AFFX-BioDn-3_at 23212.1 P 4.42873e-05
AFFX-CreX-5_at 56806.1 P 5.16732e-05
AFFX-CreX-3_at 60684.6 P 4.42873e-05
AFFX-DapX-5_at 26.5112 A 0.250796
AFFX-DapX-M_at 5.79895 A 0.824672
AFFX-DapX-3_at 5.43673 A 0.814869
AFFX-LysX-5_at 24.3745 A 0.41138
AFFX-LysX-M_at 5.01998 A 0.852061
AFFX-LysX-3_at 15.7374 A 0.588627
AFFX-PheX-5_at 4.85776 A 0.973889
AFFX-PheX-M_at 2.57149 A 0.891041
AFFX-PheX-3_at 28.8856 A 0.51489
AFFX-ThrX-5_at 34.3217 A 0.41138
AFFX-ThrX-M_at 33.3574 A 0.382599

Total number of rows: 54675

Table truncated, full table size 1630 Kbytes.

Supplementary file Size Download File type/resource
GSM692117.CEL.gz 4.5 Mb (ftp)(http) CEL
GSM692117.CHP.gz 302.2 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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