|
| Status |
Public on Mar 15, 2011 |
| Title |
Donor 4 - CSE |
| Sample type |
RNA |
| |
|
| Source name |
Human bronchial epithelial cells - 24 h CSE
|
| Organism |
Homo sapiens |
| Characteristics |
donor: 4
tissue: bronchi
cell type: epithelial cell
growth: primary cultures
stress: CSE
|
| Treatment protocol |
Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI = 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
|
| Growth protocol |
Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression in response to CSE exposure
Sample 4C
EA09124_102526_H133+_4C.CHP
EA09124_102526_H133+_4C.CEL
|
| Data processing |
The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Mar 15, 2011 |
| Last update date |
Mar 15, 2011 |
| Contact name |
David Proud |
| E-mail(s) |
dproud@ucalgary.ca
|
| Organization name |
University of Calgary
|
| Department |
Physiology & Pharmacology
|
| Lab |
HRIC 4AC60
|
| Street address |
3330 Hospital DRive NW
|
| City |
Calgary |
| State/province |
AB |
| ZIP/Postal code |
T2N 4N1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE27973 |
Human airway epithelial responses to rhinovirus infection and cigarette smoke extract alone and in combination |
|
