|
Status |
Public on Mar 15, 2011 |
Title |
Donor 4 - RV16+CSE |
Sample type |
RNA |
|
|
Source name |
Human bronchial epithelial cells - 24 h RV16+CSE
|
Organism |
Homo sapiens |
Characteristics |
donor: 4 tissue: bronchi cell type: epithelial cell growth: primary cultures stress: RV16+CSE
|
Treatment protocol |
Cells were exposed to medium alone, purified human rhinovirus type 16 (MOI = 1), cigarette smoke extract derived from research grade cigarettes, or rhinvorius + cigarette smoke extract for 24h.
|
Growth protocol |
Cells were grown on 6 well plates in Bronchial Epithelial Growth Medium (Lonza). Hydrocortisone was withdrawn from the medium for the last 24 h before stimulation. Stimulation and incubation were also performed in hydrocortisone-free medium.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extractionof total RNA was performed according to manufacturer's instruction and DNase treated.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared using the Affymetrix GeneChip 3'IVT Express kit according to manufacturer's instructions.
|
|
|
Hybridization protocol |
Following framentation, 10 µg of cRNA were hybridized for 16 hr at 45°C on Affymetrix U133 plus 2.0 human GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Gene Expression in response to RV16 in the presence of CSE Sample 4D EA09124_102527_H133+_4D.CHP EA09124_102527_H133+_4D.CEL
|
Data processing |
The data were analyzed using Expression Console Software using Affymetrix default analysis settings.
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|
|
Submission date |
Mar 15, 2011 |
Last update date |
Mar 15, 2011 |
Contact name |
David Proud |
E-mail(s) |
dproud@ucalgary.ca
|
Organization name |
University of Calgary
|
Department |
Physiology & Pharmacology
|
Lab |
HRIC 4AC60
|
Street address |
3330 Hospital DRive NW
|
City |
Calgary |
State/province |
AB |
ZIP/Postal code |
T2N 4N1 |
Country |
Canada |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE27973 |
Human airway epithelial responses to rhinovirus infection and cigarette smoke extract alone and in combination |
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