|
| Status |
Public on Dec 30, 2011 |
| Title |
WT Dox- rep2 |
| Sample type |
RNA |
| |
|
| Source name |
WT Dox- rep2
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL/6J
genotype: wild type
treatment: untreated
cell type: embryonic fibroblast
|
| Treatment protocol |
Cells were treated doxorubicin for 12 hours.
|
| Growth protocol |
Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Illumina BeadScan 500 System
|
| Description |
WT MEF cells before doxorubicin treament
|
| Data processing |
The data were normalised using quantile normalization method with beadarray version 1.10 and lumi version 1.8.3 in R/Bioconductor package.
|
| |
|
| Submission date |
Mar 16, 2011 |
| Last update date |
Dec 30, 2011 |
| Contact name |
Bhin Jin-hyuk |
| E-mail(s) |
bynjh007@postech.ac.kr
|
| Organization name |
POSTECH
|
| Department |
Department of Chemical engineering
|
| Lab |
Systems biology and Medicine
|
| Street address |
Ji-gok research center, hyoja-dong, Nam-gu
|
| City |
Pohang |
| ZIP/Postal code |
790-784 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE27983 |
Genome-wide analysis of gene expression perturbed by RORa in MEFs subjected to control and 12 hour DNA damaged condition. |
|
