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Sample GSM69458 Query DataSets for GSM69458
Status Public on Dec 15, 2005
Title CH2112
Sample type RNA
 
Source name Supraoptic nucleus_3d dehydrated
Organism Rattus norvegicus
Characteristics Strain: Sprague Dawley
Gender: Male
Age: 10-12 weeks
Tissue: Hypothalamic Supraoptic nucleus
Extracted molecule total RNA
Extraction protocol Tissue was dissected in an RNase free manner and stored in RNAlater (Ambion) at -20C for no more than 1 month. Homogenisation in QIAzol was conducted mechanically and the aqueous phase separated using a PLG column (Eppendorf). RNA was further purified using RNeasy minelute cleanup kit (Qiagen). RNA quality was confirmed using nanodrop and bioanylizer 2100 (Agilent).
Label MessageAmpII aRNA Amplification kit (Ambion)
Label protocol Protocol available at http://www.AMBION.com/catalog/CatNum.php?1753
500ng total RNA was used initially
 
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description Sample represents the supraoptic nucleus from 5 dehydrated animals (3 Days)
Data processing GCOS version 1.2
 
Submission date Aug 11, 2005
Last update date Dec 29, 2011
Contact name DAVID MURPHY
Organization name University of Bristol
Department Medicine and Dentistry
Lab Laboratories for Integrative Neuroscience and Endocrinolgy
Street address Whitson Street
City Bristol
State/province AVON
ZIP/Postal code BS1 3NY
Country United Kingdom
 
Platform ID GPL1355
Series (4)
GSE3110 Comprehensive description of the transcriptome of hypothalamo-neurohypophyseal system in euhydrated and dehydrated rat
GSE4130 The transcriptome of the hypothalamoneurohypophyseal system in euhydrated and dehydrated rats
GSE25574 Hypothalamic transcriptome plasticity in two rodent species reveals divergent differential gene expression but conserved pathways

Data table header descriptions
ID_REF
VALUE GCOS calculated signal intensity
ABS_CALL Indication of the transcript being present (P), absent (A), marginal (M)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 123.8 P 0.00011
AFFX-BioB-M_at 211 P 0.000044
AFFX-BioB-3_at 107.5 P 0.00006
AFFX-BioC-5_at 375.9 P 0.000052
AFFX-BioC-3_at 385 P 0.000052
AFFX-BioDn-5_at 723.8 P 0.00006
AFFX-BioDn-3_at 1797.5 P 0.000044
AFFX-CreX-5_at 4018 P 0.000052
AFFX-CreX-3_at 4449.7 P 0.000044
AFFX-DapX-5_at 7.2 A 0.156732
AFFX-DapX-M_at 1.4 A 0.81489
AFFX-DapX-3_at 0.9 A 0.957038
AFFX-LysX-5_at 5.3 A 0.175328
AFFX-LysX-M_at 6.7 A 0.382599
AFFX-LysX-3_at 6.2 A 0.139482
AFFX-PheX-5_at 0.6 A 0.937071
AFFX-PheX-M_at 0.8 A 0.941556
AFFX-PheX-3_at 9.4 A 0.39692
AFFX-ThrX-5_at 1.6 A 0.544587
AFFX-ThrX-M_at 3.6 A 0.574044

Total number of rows: 31099

Table truncated, full table size 815 Kbytes.




Supplementary file Size Download File type/resource
GSM69458_CH2112_rat230plus2_080605.CEL.gz 2.6 Mb (ftp)(http) CEL
Processed data not provided for this record

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