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Sample GSM69472 Query DataSets for GSM69472
Status Public on Dec 16, 2005
Title CH2117
Sample type RNA
 
Source name Paraventricular nucleus_3d dehydrated
Organism Rattus norvegicus
Characteristics Strain: Sprague Dawley
Gender: Male
Age: 10-12 weeks
Tissue: Hypothalamic Paraventricular nucleus
Extracted molecule total RNA
Extraction protocol Tissue was dissected in an RNase free manner and stored in RNAlater (Ambion) at -20C for no more than 1 month. Homogenisation in QIAzol was conducted mechanically and the aqueous phase separated using a PLG column (Eppendorf). RNA was further purified using RNeasy minelute cleanup kit (Qiagen). RNA quality was confirmed using nanodrop and bioanylizer 2100 (Agilent).
Label MessageAmpII aRNA Amplification kit (Ambion)
Label protocol Protocol available at http://www.AMBION.com/catalog/CatNum.php?1753
 
Hybridization protocol Standard Affymetrix procedures
Scan protocol Standard Affymetrix procedures
Description Sample represents the Paraventricular nucleus from 5 dehydrated animals (3 Days)
Data processing GCOS version 1.2
 
Submission date Aug 11, 2005
Last update date Dec 16, 2005
Contact name DAVID MURPHY
Organization name University of Bristol
Department Medicine and Dentistry
Lab Laboratories for Integrative Neuroscience and Endocrinolgy
Street address Whitson Street
City Bristol
State/province AVON
ZIP/Postal code BS1 3NY
Country United Kingdom
 
Platform ID GPL1355
Series (2)
GSE3111 Comprehensive description of the transcriptome of PVN in euhydrated and dehydrated rat
GSE4130 The transcriptome of the hypothalamoneurohypophyseal system in euhydrated and dehydrated rats

Data table header descriptions
ID_REF
VALUE GCOS calculated signal intensity
ABS_CALL Indication of the transcript being present (P), absent (A), marginal (M)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 92.9 P 0.00011
AFFX-BioB-M_at 150.1 P 0.000044
AFFX-BioB-3_at 72.5 P 0.000095
AFFX-BioC-5_at 300.4 P 0.000052
AFFX-BioC-3_at 280.5 P 0.000052
AFFX-BioDn-5_at 557.1 P 0.00006
AFFX-BioDn-3_at 1371.9 P 0.000044
AFFX-CreX-5_at 3247.7 P 0.000052
AFFX-CreX-3_at 3735.1 P 0.000044
AFFX-DapX-5_at 1.9 A 0.574044
AFFX-DapX-M_at 1.8 A 0.737173
AFFX-DapX-3_at 0.7 A 0.921998
AFFX-LysX-5_at 5.9 A 0.131361
AFFX-LysX-M_at 0.7 A 0.672921
AFFX-LysX-3_at 2.1 A 0.216524
AFFX-PheX-5_at 1 A 0.868639
AFFX-PheX-M_at 0.2 A 0.987453
AFFX-PheX-3_at 12.4 A 0.354453
AFFX-ThrX-5_at 0.5 A 0.897835
AFFX-ThrX-M_at 0.5 A 0.945787

Total number of rows: 31099

Table truncated, full table size 815 Kbytes.




Supplementary data files not provided

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