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Sample GSM696308 Query DataSets for GSM696308
Status Public on Dec 01, 2011
Title keratinocytes Gli1+ rep2
Sample type RNA
Source name Dorsal skin
Organism Mus musculus
Characteristics cell type: keratinocyte
genotype/variation: Gli1+
keratinocyte source: Hh responding cells
stage of hair cycle: telogen
Treatment protocol Keratinocytes from dorsal trunk skin were isolated using published methods (Jensen et al., 2010, Nat Protoc 5, 898-911).
Growth protocol Gli1-eGFP mice were housed and bred on an outcrossed Swiss Webster background in the animal facility at Memorial Sloan-Kettering Cancer Center (MSKCC) according to IACUC protocols.
Extracted molecule total RNA
Extraction protocol Trizol
Label biotin
Label protocol Nugen ovation-pico labeling
Hybridization protocol according to manufacturer
Scan protocol according to manufacturer
Description Hh responding cells
Data processing The data were analyzed with GenomeStudio version 2010.3. The data are log2 transformed and no normalization is applied.
Submission date Mar 22, 2011
Last update date Dec 01, 2011
Contact name Jeffrey Zhao
Organization name Memorial Sloan Kettering Cancer Center
Department Genomics Core
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platform ID GPL6885
Series (1)
GSE28108 Expression profile of Gli1-expressing keratinocytes from mouse skin

Data table header descriptions
VALUE log2 transformed signal intensity (no normalization applied)

Data table
ILMN_1250052 7.52126
ILMN_3122480 6.57245
ILMN_2599935 8.20071
ILMN_2675543 6.73675
ILMN_2686883 6.72339
ILMN_2751818 6.92483
ILMN_2728634 7.12259
ILMN_3040515 6.88244
ILMN_2711608 6.70511
ILMN_1232875 7.68745
ILMN_1258507 7.69792
ILMN_2746142 6.69628
ILMN_1252690 6.94976
ILMN_2655499 6.57523
ILMN_1252870 6.9985
ILMN_1248179 6.758
ILMN_2649955 7.13693
ILMN_2628708 7.3334
ILMN_3024781 6.62767
ILMN_2705628 6.76334

Total number of rows: 25697

Table truncated, full table size 524 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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