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Sample GSM696309 Query DataSets for GSM696309
Status Public on Dec 01, 2011
Title keratinocytes Gli1+ rep3
Sample type RNA
 
Source name Dorsal skin
Organism Mus musculus
Characteristics cell type: keratinocyte
genotype/variation: Gli1+
keratinocyte source: Hh responding cells
stage of hair cycle: telogen
Treatment protocol Keratinocytes from dorsal trunk skin were isolated using published methods (Jensen et al., 2010, Nat Protoc 5, 898-911).
Growth protocol Gli1-eGFP mice were housed and bred on an outcrossed Swiss Webster background in the animal facility at Memorial Sloan-Kettering Cancer Center (MSKCC) according to IACUC protocols.
Extracted molecule total RNA
Extraction protocol Trizol
Label biotin
Label protocol Nugen ovation-pico labeling
 
Hybridization protocol according to manufacturer
Scan protocol according to manufacturer
Description Hh responding cells
Data processing The data were analyzed with GenomeStudio version 2010.3. The data are log2 transformed and no normalization is applied.
 
Submission date Mar 22, 2011
Last update date Dec 01, 2011
Contact name Jeffrey Zhao
Organization name Memorial Sloan Kettering Cancer Center
Department Genomics Core
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (1)
GSE28108 Expression profile of Gli1-expressing keratinocytes from mouse skin

Data table header descriptions
ID_REF
VALUE log2 transformed signal intensity (no normalization applied)

Data table
ID_REF VALUE
ILMN_1250052 7.60756
ILMN_3122480 6.79595
ILMN_2599935 8.13155
ILMN_2675543 6.96771
ILMN_2686883 8.18966
ILMN_2751818 6.6998
ILMN_2728634 6.82413
ILMN_3040515 6.69986
ILMN_2711608 6.89791
ILMN_1232875 7.54588
ILMN_1258507 9.63259
ILMN_2746142 6.58089
ILMN_1252690 6.86613
ILMN_2655499 6.69545
ILMN_1252870 6.79726
ILMN_1248179 6.85879
ILMN_2649955 7.12053
ILMN_2628708 7.07573
ILMN_3024781 6.68504
ILMN_2705628 6.95646

Total number of rows: 25697

Table truncated, full table size 524 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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