|
| Status |
Public on Dec 01, 2011 |
| Title |
keratinocytes Gli1- CD34+ rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Dorsal skin
|
| Organism |
Mus musculus |
| Characteristics |
cell type: keratinocyte
genotype/variation: Gli1- CD34+
keratinocyte source: hair follicle bulge without Hh responding cells
stage of hair cycle: telogen
|
| Treatment protocol |
Keratinocytes from dorsal trunk skin were isolated using published methods (Jensen et al., 2010, Nat Protoc 5, 898-911).
|
| Growth protocol |
Gli1-eGFP mice were housed and bred on an outcrossed Swiss Webster background in the animal facility at Memorial Sloan-Kettering Cancer Center (MSKCC) according to IACUC protocols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
Nugen ovation-pico labeling
|
| |
|
| Hybridization protocol |
according to manufacturer
|
| Scan protocol |
according to manufacturer
|
| Description |
hair follicle bulge without Hh responding cells
|
| Data processing |
The data were analyzed with GenomeStudio version 2010.3. The data are log2 transformed and no normalization is applied.
|
| |
|
| Submission date |
Mar 22, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Jeffrey Zhao |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Genomics Core
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE28108 |
Expression profile of Gli1-expressing keratinocytes from mouse skin |
|
