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Sample GSM696315 Query DataSets for GSM696315
Status Public on Dec 01, 2011
Title keratinocytes Gli1- CD34- rep3
Sample type RNA
 
Source name Dorsal skin
Organism Mus musculus
Characteristics cell type: keratinocyte
genotype/variation: Gli1- CD34-
keratinocyte source: primarily interfollicular epidermis and follicle infundibulum
stage of hair cycle: telogen
Treatment protocol Keratinocytes from dorsal trunk skin were isolated using published methods (Jensen et al., 2010, Nat Protoc 5, 898-911).
Growth protocol Gli1-eGFP mice were housed and bred on an outcrossed Swiss Webster background in the animal facility at Memorial Sloan-Kettering Cancer Center (MSKCC) according to IACUC protocols.
Extracted molecule total RNA
Extraction protocol Trizol
Label biotin
Label protocol Nugen ovation-pico labeling
 
Hybridization protocol according to manufacturer
Scan protocol according to manufacturer
Description primarily interfollicular epidermis and follicle infundibulum
Data processing The data were analyzed with GenomeStudio version 2010.3. The data are log2 transformed and no normalization is applied.
 
Submission date Mar 22, 2011
Last update date Dec 01, 2011
Contact name Jeffrey Zhao
Organization name Memorial Sloan Kettering Cancer Center
Department Genomics Core
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (1)
GSE28108 Expression profile of Gli1-expressing keratinocytes from mouse skin

Data table header descriptions
ID_REF
VALUE log2 transformed signal intensity (no normalization applied)

Data table
ID_REF VALUE
ILMN_1250052 7.44733
ILMN_3122480 6.66769
ILMN_2599935 8.66338
ILMN_2675543 6.89874
ILMN_2686883 7.65998
ILMN_2751818 6.90596
ILMN_2728634 7.12307
ILMN_3040515 6.85358
ILMN_2711608 6.94422
ILMN_1232875 7.72125
ILMN_1258507 8.64658
ILMN_2746142 6.87761
ILMN_1252690 6.98315
ILMN_2655499 6.95764
ILMN_1252870 6.99427
ILMN_1248179 6.88513
ILMN_2649955 6.99211
ILMN_2628708 7.53413
ILMN_3024781 6.72059
ILMN_2705628 6.76458

Total number of rows: 25697

Table truncated, full table size 524 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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