|
| Status |
Public on Apr 01, 2011 |
| Title |
pSuper vector control 2 |
| Sample type |
RNA |
| |
|
| Source name |
pSuper vector control 2
|
| Organism |
Mus musculus |
| Characteristics |
cell line: E14
treatment: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 23, 2011 |
| Last update date |
Apr 01, 2011 |
| Contact name |
hongbing yu |
| E-mail(s) |
yuhb@gis.a-star.edu.sg
|
| Phone |
65 68088488
|
| Fax |
65 68088306
|
| Organization name |
genome institute of singapore
|
| Department |
stem cell and development
|
| Lab |
stem cell 3
|
| Street address |
60 biopolis street, #02-01, genome
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE28141 |
Genome-wide analysis of REST knockdown responsive gene expression in mouse ES cells |
| GSE28289 |
REST ChIP-chip and knockdown expression profiling |
|
