|
| Status |
Public on Sep 25, 2011 |
| Title |
Untreated HAND brain 4, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Homo sapiens postmortem brain tissue
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: untreated
tissue: postmortem brain
disease state: HIV1 with HIV-1-associated neurocognitive disorders
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from human brain tissue by RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling using NuGen Encore Biotin Module.
|
| |
|
| Hybridization protocol |
The samples were hybridized to Affymetrix Human Genome 133 plus 2.0 arrays. Washing and staining of all arrays were carried out in the Affymetrix fluidics module as per the manufacturer's protocol.
|
| Scan protocol |
The detection and quantitation of target hybridization was performed with an Affymetrix GeneChip Scanner.
|
| Description |
gene expression data for portmortem brain tissue uninfected patients
|
| Data processing |
The .cel data files generated by the Affymetrix microarray hybridization platform were analyzed by the ArrayAssist software (Stratagene, Santa Clara, CA). Probe level analysis was performed using the Robust Microarray Average (RMA) algorithm.
|
| |
|
| Submission date |
Mar 24, 2011 |
| Last update date |
Sep 25, 2011 |
| Contact name |
Alejandra Borjabad |
| E-mail(s) |
ab2612@columbia.edu
|
| Phone |
9175574628
|
| Organization name |
St.Luke's-Roosevelt Hospital Center
|
| Department |
Medicine
|
| Lab |
Molecular Virology
|
| Street address |
432 West 58th, Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10019 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28160 |
Significant Effects of Antiretroviral Therapy on Global Gene Expression in Brain Tissues of Patients with HIV-Associated Neurocognitive Disorders |
|
