NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM699659 Query DataSets for GSM699659
Status Public on Mar 30, 2011
Title LY2_6hr_100nM-4-OHT_3
Sample type other
 
Channel 1
Source name Breast Cancer Cells treated with EtOH, E2, or 4-OHT
Organism Homo sapiens
Characteristics gender: female
cell line: LY2 breast cancer cell line
tamoxifen sensitivity: resistant
agent: 4-OHT
Treatment protocol Cells were treated with Ethanol (EtOH- vehicle control), 10 nM estradiol (E2), or 100 nM 4-hydroxytamoxifen (4-OHT) for 6 hr
Growth protocol MCF-7 cells were grown in IMEM with 5% FBS + 1% PS and were serum starved (5% dextran-coated charcoal stripped FBS in IMEM phenol red free medium) for 48 h prior to treatment
Extracted molecule other
Extraction protocol RNA was extracted using miRVANA miRNA isolation kit from Ambion
Label Hy3
Label protocol Cells were serum-starved for 48 h prior to 6 h treatment with Ethanol (EtOH- vehicle control), 10 nM estradiol (E2), or 100 nM 4-hydroxytamoxifen (4-OHT)
 
Channel 2
Source name Exiqon Common Reference Pool
Organism Homo sapiens
Characteristics reference: Exiqon Common Reference Pool
Treatment protocol Cells were treated with Ethanol (EtOH- vehicle control), 10 nM estradiol (E2), or 100 nM 4-hydroxytamoxifen (4-OHT) for 6 hr
Growth protocol MCF-7 cells were grown in IMEM with 5% FBS + 1% PS and were serum starved (5% dextran-coated charcoal stripped FBS in IMEM phenol red free medium) for 48 h prior to treatment
Extracted molecule other
Extraction protocol RNA was extracted using miRVANA miRNA isolation kit from Ambion
Label Hy5
Label protocol Cells were serum-starved for 48 h prior to 6 h treatment with Ethanol (EtOH- vehicle control), 10 nM estradiol (E2), or 100 nM 4-hydroxytamoxifen (4-OHT)
 
 
Hybridization protocol Samples were sent to Exiqon where they were labeled and hybridized into the miRCURY LNA microarray (miRbase 11.0 human array)
Scan protocol MiRNA analysis was performed by Exiqon
Description CH1 raw data file: 1_Exiqon_13973523_S01_Cropped.txt
CH2 raw data file: 0_Exiqon_13973523_S01_Cropped.txt
Data processing LOESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Exiqon performed these calculations/analyses.
 
Submission date Mar 30, 2011
Last update date Mar 30, 2011
Contact name Carolyn M Klinge
E-mail(s) carolyn.klinge@louisville.edu
Organization name University of Louisville School of Medicine
Department Biochemistry & Molecular Biology
Street address 580 S Preston St
City Louisville
State/province KY
ZIP/Postal code 40202
Country USA
 
Platform ID GPL7723
Series (1)
GSE28267 Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells

Data table header descriptions
ID_REF
VALUE log2(Hy3/Hy5) Log Median Ratios (combined signal from both channels)- calculation was performed by Exiqon

Data table
ID_REF VALUE
17748 -0.052294609
17888 0.464987754
17749 0.382096275
42769 0.047778166
19004 0.325749054
42653 -0.013648361
46437 -0.084393368
42505 0.290851232
46436 0.138553912
42743 -0.07514121
46435 -0.120106599
42768 -0.104547499
42742 0.0984006
46438 0.07370143
42778 0.168167005
9938 0.518469034
42534 -0.516366544
10916 -0.429384163
19581 1.065506833
42563 -0.118929182

Total number of rows: 1983

Table truncated, full table size 35 Kbytes.




Supplementary file Size Download File type/resource
GSM699659_0_Exiqon_13973523_S01_Cropped.txt.gz 799.9 Kb (ftp)(http) TXT
GSM699659_1_Exiqon_13973523_S01_Cropped.txt.gz 876.0 Kb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap