|
| Status |
Public on Mar 30, 2011 |
| Title |
LY2_6hr_100nM-4-OHT_4 |
| Sample type |
other |
| |
|
| Channel 1 |
| Source name |
Breast Cancer Cells treated with EtOH, E2, or 4-OHT
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
cell line: LY2 breast cancer cell line
tamoxifen sensitivity: resistant
agent: 4-OHT
|
| Treatment protocol |
Cells were treated with Ethanol (EtOH- vehicle control), 10 nM estradiol (E2), or 100 nM 4-hydroxytamoxifen (4-OHT) for 6 hr
|
| Growth protocol |
MCF-7 cells were grown in IMEM with 5% FBS + 1% PS and were serum starved (5% dextran-coated charcoal stripped FBS in IMEM phenol red free medium) for 48 h prior to treatment
|
| Extracted molecule |
other |
| Extraction protocol |
RNA was extracted using miRVANA miRNA isolation kit from Ambion
|
| Label |
Hy3
|
| Label protocol |
Cells were serum-starved for 48 h prior to 6 h treatment with Ethanol (EtOH- vehicle control), 10 nM estradiol (E2), or 100 nM 4-hydroxytamoxifen (4-OHT)
|
| |
|
| Channel 2 |
| Source name |
Exiqon Common Reference Pool
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Exiqon Common Reference Pool
|
| Treatment protocol |
Cells were treated with Ethanol (EtOH- vehicle control), 10 nM estradiol (E2), or 100 nM 4-hydroxytamoxifen (4-OHT) for 6 hr
|
| Growth protocol |
MCF-7 cells were grown in IMEM with 5% FBS + 1% PS and were serum starved (5% dextran-coated charcoal stripped FBS in IMEM phenol red free medium) for 48 h prior to treatment
|
| Extracted molecule |
other |
| Extraction protocol |
RNA was extracted using miRVANA miRNA isolation kit from Ambion
|
| Label |
Hy5
|
| Label protocol |
Cells were serum-starved for 48 h prior to 6 h treatment with Ethanol (EtOH- vehicle control), 10 nM estradiol (E2), or 100 nM 4-hydroxytamoxifen (4-OHT)
|
| |
|
| |
| Hybridization protocol |
Samples were sent to Exiqon where they were labeled and hybridized into the miRCURY LNA microarray (miRbase 11.0 human array)
|
| Scan protocol |
MiRNA analysis was performed by Exiqon
|
| Description |
CH1 raw data file: 1_Exiqon_13973524_S01_Cropped.txt
CH2 raw data file: 0_Exiqon_13973524_S01_Cropped.txt
|
| Data processing |
LOESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Exiqon performed these calculations/analyses.
|
| |
|
| Submission date |
Mar 30, 2011 |
| Last update date |
Mar 30, 2011 |
| Contact name |
Carolyn M Klinge |
| E-mail(s) |
carolyn.klinge@louisville.edu
|
| Organization name |
University of Louisville School of Medicine
|
| Department |
Biochemistry & Molecular Biology
|
| Street address |
580 S Preston St
|
| City |
Louisville |
| State/province |
KY |
| ZIP/Postal code |
40202 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE28267 |
Differential expression of microRNA expression in tamoxifen-sensitive MCF-7 versus tamoxifen-resistant LY2 human breast cancer cells |
|
