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Sample GSM701838 Query DataSets for GSM701838
Status Public on Apr 06, 2011
Title Aire knock out 1
Sample type RNA
 
Source name MHCIIhi mTECs
Organism Mus musculus
Characteristics strain: C57Bl/6
genotype: ako
beadchip: 1
Sex: male
date: Oct-08
Extracted molecule total RNA
Extraction protocol RNA was isolated using a Qiagen RNeasy mini kit including an on column DNase digest as per manufacturer's instructions.
Label Cy3
Label protocol Standard Illumina protocol
 
Hybridization protocol Standard Illumina protocol
Scan protocol Standard Illumina protocol
Data processing Un-normalized summary probe profiles were output from BeadStudio for two Illumina Mouse-6 Expression Beadchip (v1.1). The raw intensities were background corrected using the negative controls, a variance stabilising transformation (vst) was applied and the data quantile normalised using all the arrays on the two beadchips using the lumi package of the Bioconductor open-source software project (http://www.bioconductor.org).
 
Submission date Apr 05, 2011
Last update date Apr 06, 2011
Contact name Francois-Xavier Hubert
E-mail(s) hubert@wehi.edu.au
Organization name The Walter and Eliza Hall Institute of Medical Research
Department Immunology
Street address 1G Royal Parade
City Melbourne
State/province Victoria
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL6481
Series (1)
GSE28393 Aire regulates transfer of antigen from mTEC to dendritic cells for induction of thymic tolerance

Data table header descriptions
ID_REF
VALUE lumi-software computed normalized log2-intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
102450270 6.257904149 0.46288
104200035 6.210263912 0.35995
100730138 6.525350817 0.78665
104230390 6.817630817 0.92639
102630735 6.31134463 0.55583
100450435 6.540243758 0.79663
101230594 6.162233489 0.26326
100780541 6.284717292 0.51778
104200184 6.098459203 0.1753
105360286 6.172620953 0.28384
103060411 6.365203946 0.62944
100630193 6.172620953 0.29008
105270286 6.052285782 0.11915
101690685 6.33587358 0.59077
100060180 6.308935375 0.55022
107100563 6.354892772 0.62009
100450451 6.068692791 0.13787
100610092 6.124523472 0.20649
104810452 14.16090897 1
6840019 12.60370334 1

Total number of rows: 46619

Table truncated, full table size 1257 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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