|
Status |
Public on Dec 01, 2011 |
Title |
Zebrafish NMLF at 4h after surgery, sham control 2 |
Sample type |
RNA |
|
|
Source name |
Zebrafish brain, laser capture microdissected NMLF region
|
Organism |
Danio rerio |
Characteristics |
treatment: Control time: 4h
|
Treatment protocol |
Laser capture microdissection, using the PALM system (Carl Zeiss).
|
Growth protocol |
Zebrafish were kept in aquaria with a 14-hour light and 10-hour dark cycle at 28C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Microdissected tissues were collected in Qiagen buffer RLT and processed with the RNeasy Micro kit. RNA samples were amplified using the RampUP kit (Genisphere).
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocols were used for preparation of bio-cDNA.
|
|
|
Hybridization protocol |
Standard Affymetrix protocols were used for hybridization.
|
Scan protocol |
Standard Affymetrix protocols were used for scanning.
|
Data processing |
CHP and CEL files were produced using Affymetrix software. Programmatic identifier: GeneChip.CallGEBaseCall.1 Algorithm: ExpressionStat 5.0
|
|
|
Submission date |
Apr 08, 2011 |
Last update date |
Dec 01, 2011 |
Contact name |
Ronald P. Hart |
E-mail(s) |
rhart@rutgers.edu
|
Phone |
848-445-1783
|
Organization name |
Rutgers University
|
Department |
Cell Biology & Neuroscience
|
Street address |
604 Allison Rd Rm B430
|
City |
Piscataway |
State/province |
NJ |
ZIP/Postal code |
08854 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE28470 |
NMLF response to spinal cord injury in Zebrafish |
|