NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM703420 Query DataSets for GSM703420
Status Public on Dec 01, 2011
Title Zebrafish NMLF at 4h after surgery, sham control 2
Sample type RNA
 
Source name Zebrafish brain, laser capture microdissected NMLF region
Organism Danio rerio
Characteristics treatment: Control
time: 4h
Treatment protocol Laser capture microdissection, using the PALM system (Carl Zeiss).
Growth protocol Zebrafish were kept in aquaria with a 14-hour light and 10-hour dark cycle at 28C.
Extracted molecule total RNA
Extraction protocol Microdissected tissues were collected in Qiagen buffer RLT and processed with the RNeasy Micro kit. RNA samples were amplified using the RampUP kit (Genisphere).
Label biotin
Label protocol Standard Affymetrix protocols were used for preparation of bio-cDNA.
 
Hybridization protocol Standard Affymetrix protocols were used for hybridization.
Scan protocol Standard Affymetrix protocols were used for scanning.
Data processing CHP and CEL files were produced using Affymetrix software.
Programmatic identifier: GeneChip.CallGEBaseCall.1
Algorithm: ExpressionStat 5.0
 
Submission date Apr 08, 2011
Last update date Dec 01, 2011
Contact name Ronald P. Hart
E-mail(s) rhart@rutgers.edu
Phone 848-445-1783
Organization name Rutgers University
Department Cell Biology & Neuroscience
Street address 604 Allison Rd Rm B430
City Piscataway
State/province NJ
ZIP/Postal code 08854
Country USA
 
Platform ID GPL1319
Series (1)
GSE28470 NMLF response to spinal cord injury in Zebrafish

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 730.234 P 0.000195116
AFFX-BioB-M_at 1130.84 P 8.14279e-05
AFFX-BioB-3_at 889.927 P 0.00179591
AFFX-BioC-5_at 1643.92 P 9.4506e-05
AFFX-BioC-3_at 948.831 P 0.000581214
AFFX-BioDn-5_at 3310.17 P 4.42873e-05
AFFX-BioDn-3_at 6482.12 P 4.42873e-05
AFFX-CreX-5_at 11854.6 P 4.42873e-05
AFFX-CreX-3_at 18141.3 P 4.42873e-05
AFFX-DapX-5_at 7.4592 A 0.617401
AFFX-DapX-M_at 3.97843 A 0.984817
AFFX-DapX-3_at 8.54648 A 0.834139
AFFX-LysX-5_at 9.35164 A 0.485105
AFFX-LysX-M_at 2.8438 A 0.969024
AFFX-LysX-3_at 0.651658 A 0.99156
AFFX-PheX-5_at 2.76496 A 0.937071
AFFX-PheX-M_at 4.65522 A 0.932322
AFFX-PheX-3_at 34.1459 A 0.41138
AFFX-ThrX-5_at 16.2039 A 0.382599
AFFX-ThrX-M_at 2.86829 A 0.883887

Total number of rows: 15617

Table truncated, full table size 564 Kbytes.




Supplementary file Size Download File type/resource
GSM703420.CEL.gz 1.9 Mb (ftp)(http) CEL
GSM703420.CHP.gz 92.0 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap