|
| Status |
Public on Dec 01, 2011 |
| Title |
Zebrafish NMLF at 4h after surgery, spinal cord transected 2 |
| Sample type |
RNA |
| |
|
| Source name |
Zebrafish brain, laser capture microdissected NMLF region
|
| Organism |
Danio rerio |
| Characteristics |
treatment: Injured
time: 4h
|
| Treatment protocol |
Laser capture microdissection, using the PALM system (Carl Zeiss).
|
| Growth protocol |
Zebrafish were kept in aquaria with a 14-hour light and 10-hour dark cycle at 28C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Microdissected tissues were collected in Qiagen buffer RLT and processed with the RNeasy Micro kit. RNA samples were amplified using the RampUP kit (Genisphere).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocols were used for preparation of bio-cDNA.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols were used for hybridization.
|
| Scan protocol |
Standard Affymetrix protocols were used for scanning.
|
| Data processing |
CHP and CEL files were produced using Affymetrix software.
Programmatic identifier: GeneChip.CallGEBaseCall.1
Algorithm: ExpressionStat 5.0
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Ronald P. Hart |
| E-mail(s) |
rhart@rutgers.edu
|
| Phone |
848-445-1783
|
| Organization name |
Rutgers University
|
| Department |
Cell Biology & Neuroscience
|
| Street address |
604 Allison Rd Rm B430
|
| City |
Piscataway |
| State/province |
NJ |
| ZIP/Postal code |
08854 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE28470 |
NMLF response to spinal cord injury in Zebrafish |
|
