|
| Status |
Public on Jan 23, 2012 |
| Title |
CD4+ T cells rep2 miRNA (Roche) |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells from healthy donor pool 4
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4+ T cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA, including small non-coding RNAs, was extracted using the mirVana RNA isolation kit (Ambion, Inc, Austin, TX, USA) according to manufacturer’s protocol. RNA was quantified using a ND-1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK) and RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent technologies, Santa Clara, CA, USA). The 260/280 ratio were between 1.7 and 2.1 and the RINs (RNA Integrity Numbers) greater than 6 for all samples.
|
| Label |
Biotin
|
| Label protocol |
Total RNA (300ng) was biotin labeled using the FlashTag™ HSR RNA labeling kit for Affymetrix miRNA arrays (Genisphere LLC., Hatfield, PA, USA) according to manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to Affymetrix GeneChip miRNA microarrays according to manufacturer’s instructions. Wash and stain protocol FS450_0003.
|
| Scan protocol |
GeneChips were scanned using the GC 30007G scanner.
|
| Description |
CD4+ T cells were isolated from the monocytes-depleted fraction (obtained during the monocytes isolation) using the EasySep® Human CD4 Positive Selection Kit (Stemcell Technologies) according to manufacturer’s instructions.
|
| Data processing |
Data was normalized in R/Bioconductor using the robust multi-array average (RMA) expression measure.
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Jan 24, 2012 |
| Contact name |
Donavan Cheng |
| E-mail(s) |
donavan.cheng@gmail.com
|
| Organization name |
Hoffmann-LaRoche
|
| Department |
Translational Research Sciences
|
| Street address |
340 Kingsland Ave
|
| City |
Nutley |
| State/province |
NJ |
| ZIP/Postal code |
07110 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE28487 |
miRNA expression profiling of human immune cell subsets (Roche) |
| GSE28492 |
miRNA and mRNA expression profiling of human immune cell subsets |
|
